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Protective Role of a methanolic Extract of Spinach (Spinacia oleracea L.) Against Adverse Effects of UV-C Irradiation on Fenugreek (Trigonella foenum-graecum L.) Seedlings

Die protektive Rolle eines methanolischen Spinatextraktes (Spinacia oleracea L.) gegen Nebenwirkungen der UV‑C-Bestrahlung auf Bockhornkleesprosse (Trigonella foenum-graecum L.).

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Abstract

Photosynthesis is an important process for plants in which plants utilize the energy from sunlight to manufacture and produce all their own food. Although UV-C damage is not physiologically relevant for plants growing in the sun, short-wavelength (UV-C) radiation from germicidal lamps has been used to study cell damage in animals as well as in plants. This study provides a review of the effects of a methanolic extract of spinach (SE) as a natural powerful antioxidants used at two concentrations (25 and 50 ppm) on fenugreek seedlings grown under UV-C stress conditions. The results revealed that exposure of fenugreek seedlings to low UV-C period (30 min) increased growth criteria of the produced seedlings. In addition, the contents of chlorophyll, total pigments, total protein, free amino acids, non-enzymatic (carotenoids, total phenol, flavonoids, α‑tocopherol & ascorbic acid contents) and enzymatic antioxidants were increased at this period. By increasing exposure periods of UV-C radiation, the growth criteria and chemical constitutes of the seedlings were decreased. Application of lower concentration of SE (25 ppm) improve the growth of the seedlings at 30-min UV-C and was able to protect seedlings from high periods of UV-C irradiation by increasing all measured parameters except CAT. These increases might be an adaptive mechanism to minimize the adverse effects of longer exposure of UV-C radiation. Moreover, treating the irradiated seedlings with spinach extract led to an obvious alteration in gene expression including synthesis of some proteins bands and disappearance of other protein sets of the protein profile.

Zusammenfassung

Die Photosynthese ist ein wichtiger Prozess für Pflanzen, bei dem sie die Sonnenenergie zum Aufbau und zur Erzeugung ihrer lebensnotwendigen Stoffe benutzen. Obwohl die UV-C-Schäden physiologisch für die in der Sonne gewachsenen Pflanzen nicht relevant sind, wurde die Kurzwellenradiation (UV-C) aus Ultraviolett-Lampen verwendet, um Zellenschäden bei Tieren und Pflanzen zu untersuchen.

Diese Studie setzt sich mit den Resultaten über die Wirkung des Spinatextraktes (SE) als natürliches, starke Antioxidationsmittel in zwei Konzentrationen (25 und 50 ppm) auf die Bockhornkleesprossen, die unter belastenden UV-C-Bestrahlungen aufgewachsen sind, auseinander. Die Ergebnisse zeigen, dass die Bestrahlung der Bockhornkleesprosse mit UV-C für eine kurze Zeitdauer (30 min) die Wachstumsmerkmale der produzierten Sprosse erweitert. Außerdem erhöht sich der Gehalt an Chlorophyll, Gesamtpigmenten, Gesamtprotein, freien Aminosäuren, nicht-enzymatischen (Karotinide, Gesamtphenole, Flavonoide, Alpha-Tocopherol und Ascorbicsäure) und enzymatischen Antioxidationsmitteln in dieser Periode. Bei einer Verlängerung der Bestrahlungszeit mit UV-C verringern sich die Wachstumsmerkmale und die chemischen Bestandteile der Sprosse. Die Verwendung der niedrigen SE-Konzentration (25 ppm) verbessert die Wachstumsmerkmale der Sprosse bei 30-Minuten-UV-C-Bestrahlung und schützt die Sprosse vor langen Perioden der UV-C-Irradiation. Das zeigt sich an der Steigerung aller Messparameter außer CAT. Diese Steigerung könnte ein Kompensationsmechanismus zur Reduzierung der schlechten Einflüsse der langen UV-C-Radiation sein. Außerdem führt die Behandlung der bestrahlten Sprosse mit dem Spinatextrakt zu einer klaren Veränderung in der Genexpression: zum einen in der Synthese sowie sowie im Verschwinden Proteingruppen in dem Proteinprofil.

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  1. Biological and Geological Sciences Department, Faculty of Education, Ain Shams University El Makres St. Roxy, 1575, Cairo, Egypt

    Abdel Haleem Mohammed Ahmed Mohammed & Samia Ageeb Akladious

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  1. Abdel Haleem Mohammed Ahmed Mohammed
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Correspondence to Samia Ageeb Akladious.

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A.H.M.A. Mohammed and S.A. Akladious declare that they have no competing interests.

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Mohammed, A.H.M.A., Akladious, S.A. Protective Role of a methanolic Extract of Spinach (Spinacia oleracea L.) Against Adverse Effects of UV-C Irradiation on Fenugreek (Trigonella foenum-graecum L.) Seedlings. Gesunde Pflanzen 69, 185–196 (2017). https://doi.org/10.1007/s10343-017-0404-x

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