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Characterization and Application of Membrane-emulsified HLB Microspheres for Quantification of 25-Hydroxy Vitamin D2 and 25-Hydroxy Vitamin D3 in Serum by UPLC-MS/MS

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Abstract

Membrane emulsification can obtain microspheres with relatively uniform particle size and pore size, which has unique advantages in the synthesis of polymer microsphere adsorbents and the enrichment and separation of organic compounds. In this study, the Hydrophilic Lipophilic Balance (HLB) solid phase extraction microspheres were prepared by membrane emulsification technique and was used in the pretreatment of samples to detect 25-hydroxy vitamin D2 (25OH-VD2) and 25-hydroxy vitamin D3 (25OH-VD3) in serum by UPLC-MS/MS for the first time. To confirm the efficacy of membrane-emulsified (ME) HLB microspheres, three types of microspheres, including ME HLB microspheres, non-membrane-emulsified (non-ME) HLB microspheres and commercial HLB microspheres, were compared for the pretreatment of 25OH-VD2 and 25OH-VD3. The performance of HLB microspheres was characterized based on the sample recovery values acquired by UPLC-MS/MS analysis. Results showed that among the three types of microspheres, ME HLB microspheres showed the best performance and demonstrated good adsorption properties for 25OH-VD2 and 25OH-VD3. The recoveries range of 25OH-VD2 and 25OH-VD3 were 96.7–101.4 and 98.7–104.9%, respectively, and the coefficients of variation were 0.67–1.24 and 1.39–2.28%, respectively, which were superior to those of commercial HLB microspheres and non-ME HLB microspheres. Additionally, due to their excellent homogeneity, the ME HLB microspheres exhibited good precision in the determination of low values of 25OH-VD2 and 25OH-VD3, indicating accurate and simple detection of 25OH-VD2 and 25OH-VD3 with a broad potential for further development.

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No datasets were generated or analysed during the current study.

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Acknowledgements

This work was financially supported by Epsilon Biotech. Co. (Zhejiang, China), National Natural Science Foundation of China (Grant No. 81402889), 131 innovative talents training project in Tianjin, Scientific Research and Development Center of Higher Education of Ministry of Education “Innovative Application of Virtual Simulation Technology in Vocational Education Teaching” special project: “Research on the construction path and effect of Virtual simulation training Base for Health vocational Education”, project number: ZJXF2022094.

Funding

National Natural Science Foundation of China, 81402889.

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Author notes

  1. Jihua Zhang, Zishan Gong have contributed equally to this work.

Authors and Affiliations

  1. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, People’s Republic of China

    Jihua Zhang, Hang Tie, Xuan Wang & Liang Xu

  2. Tianjin Anding Hospital, Mental Health Center of Tianjin Medical University, Institute of Mental Health, Tianjin, 300222, People’s Republic of China

    Jihua Zhang

  3. Department of Pharmacy and Medical Examination Technology, Tianjin Medical College, Tianjin, 300222, People’s Republic of China

    Zishan Gong, Weixiang Zhai, Qiaoyun Guo, Wenhui Wu, Jiyang Liu, Liang Xu, Wenli Jing & Shuo Zhang

  4. Chinese Academy of Inspection and Quarantine Greater Bay Area, Guangdong, 528400, People’s Republic of China

    Hang Tie

  5. The Second Hospital of Tianjin Medical University, Tianjin, 300211, People’s Republic of China

    Yanchun Wang

  6. Epsilon Biotechnology Corporation, Zhejiang, 311222, People’s Republic of China

    Jiyang Liu

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  1. Jihua Zhang
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Contributions

Jihua, Zishan and Xuan wrote the main manuscript text. Hang and Yanchun prepared Figs. 1, 2, 3, and 4. Weixiang and Qiaoyun prepared Figs. 5 and 6. All authors reviewed the manuscript.

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Correspondence to Liang Xu.

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Zhang, J., Gong, Z., Tie, H. et al. Characterization and Application of Membrane-emulsified HLB Microspheres for Quantification of 25-Hydroxy Vitamin D2 and 25-Hydroxy Vitamin D3 in Serum by UPLC-MS/MS. Chromatographia 87, 351–361 (2024). https://doi.org/10.1007/s10337-024-04331-1

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