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Improved Method for Stratum Corneum Lipid Analysis by Automated Multiple Development HPTLC

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Abstract

An improved high-performance thin-layer chromatographic (HPTLC) method has been developed for quantitative analysis of stratum corneum lipids. When used for quantification of in-vivo-extracted skin lipids, the optimized method resulted in improved separation of cholesterol, cholesteryl esters, free fatty acids, and squalene. Variation in repeatability for cholesterol, cholesteryl esters and free fatty acids was under ±5%. The extraction recovery was 91.4 ± 1.2% (mean ± CV). Coefficients of variation ranged from 0.8 to 3.8%. The limits of detection S/N 3:1 and of quantification S/N 5:1 under the given conditions were: cholesteryl sulfate 12.5/25 ng, ceramide [AP]D-compound 50/250 ng, ceramide [NP] 50/125 ng, ceramide [NS] 125/250 ng, cholesterol 50/125 ng, palmitic acid 50/250 ng, and cholesteryl oleate 50/125 ng.

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Acknowledgments

The authors would like to thank Anke Nies for her technical assistance.

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Authors and Affiliations

  1. Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle, Germany

    Annett Opitz & Reinhard H. H. Neubert

  2. Cognis GmbH, Düsseldorf, Germany

    Michaela Wirtz, David Melchior, Annette Mehling & Hans-Willi Kling

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  1. Annett Opitz
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  2. Michaela Wirtz
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  3. David Melchior
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  4. Annette Mehling
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  5. Hans-Willi Kling
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  6. Reinhard H. H. Neubert
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Correspondence to Reinhard H. H. Neubert.

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Opitz, A., Wirtz, M., Melchior, D. et al. Improved Method for Stratum Corneum Lipid Analysis by Automated Multiple Development HPTLC. Chromatographia 73, 559–565 (2011). https://doi.org/10.1007/s10337-011-1913-x

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  • DOI: https://doi.org/10.1007/s10337-011-1913-x

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