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Enhanced microbial lipid production by Cryptococcus albidus in the high-cell-density continuous cultivation with membrane cell recycling and two-stage nutrient limitation

  • Fermentation, Cell Culture and Bioengineering - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

As a potential feedstock for biofuel production, a high-cell-density continuous culture for the lipid production by Cryptococcus albidus was investigated in this study. The influences of dilution rates in the single-stage continuous cultures were explored first. To reach a high-cell-density culture, a single-stage continuous culture coupled with a membrane cell recycling system was carried out at a constant dilution rate of 0.36/h with varied bleeding ratios. The maximum lipid productivity of 0.69 g/L/h was achieved with the highest bleeding ratio of 0.4. To reach a better lipid yield and content, a two-stage continuous cultivation was performed by adjusting the C/N ratio in two different stages. Finally, a lipid yield of 0.32 g/g and lipid content of 56.4% were obtained. This two-stage continuous cultivation, which provided a higher lipid production performance, shows a great potential for an industrial-scale biotechnological production of microbial lipids and biofuel production.

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Acknowledgments

This work was supported by the Key Research and Development Program of Shaanxi Province (2017GY-146), the China postdoctoral science foundation (2017M623206), and the National Research Foundation of Korea Grant (NRF-2011-0009582).

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Authors and Affiliations

  1. School of Chemical Engineering, Northwest University, Xi’an, 710069, China

    Rongzhan Fu

  2. School of Chemical Engineering and Technology, Xi’an Jiaotong University, Xi’an, 710049, China

    Qiang Fei

  3. College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo, 315100, China

    Longan Shang

  4. Department of Bioengineering, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA

    Christopher J. Brigham

  5. Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701, Korea

    Ho Nam Chang

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  1. Rongzhan Fu
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  2. Qiang Fei
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  3. Longan Shang
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  4. Christopher J. Brigham
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  5. Ho Nam Chang
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Correspondence to Qiang Fei.

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Fu, R., Fei, Q., Shang, L. et al. Enhanced microbial lipid production by Cryptococcus albidus in the high-cell-density continuous cultivation with membrane cell recycling and two-stage nutrient limitation. J Ind Microbiol Biotechnol 45, 1045–1051 (2018). https://doi.org/10.1007/s10295-018-2081-x

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  • DOI: https://doi.org/10.1007/s10295-018-2081-x

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