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Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from Streptomyces lavendulae FRI-5

  • Genetics and Molecular Biology of Industrial Organisms - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The genome of streptomycetes has the ability to produce many novel and potentially useful bioactive compounds, but most of which are not produced under standard laboratory cultivation conditions and are referred to as silent/cryptic secondary metabolites. Streptomyces lavendulae FRI-5 produces several types of bioactive compounds. However, this strain may also have the potential to biosynthesize more useful secondary metabolites. Here, we activated a silent biosynthetic gene cluster of an uncharacterized compound from S. lavendulae FRI-5 using heterologous expression. The engineered strain carrying the silent gene cluster produced compound 5, which was undetectable in the culture broth of S. lavendulae FRI-5. Using various spectroscopic analyses, we elucidated the chemical structure of compound 5 (named lavendiol) as a new diol-containing polyketide. The proposed assembly line of lavendiol shows a unique biosynthetic mechanism for polyketide compounds. The results of this study suggest the possibility of discovering more silent useful compounds from streptomycetes by genome mining and heterologous expression.

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Acknowledgements

This work was supported by a Grant-in-Aid for Scientific Research (C) (Grant Number JP15K07358) from the Japan Society for the Promotion of Science (JSPS) to S.K., by the New Chemical Technology Research Encouragement Award from the Japan Association for Chemical Innovation to S.K., and by a scholarship from the Ministry of Education, Culture, Sports, Science, and Technology of Japan to I.G.U.P.

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Authors and Affiliations

  1. International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Ivy Grace Umadhay Pait, Shigeru Kitani, Farah Wahidah Roslan, Dana Ulanova & Takuya Nihira

  2. Department of Marine Resource Science, Faculty of Agriculture and Marine Science, Kochi University, 200 Otsu, Monobe, Nankoku, Kochi, 783-8502, Japan

    Dana Ulanova

  3. Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Masayoshi Arai

  4. Kitasato Institute for Life Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa, 252-0373, Japan

    Haruo Ikeda

  5. MU-OU Collaborative Research Center for Bioscience and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd, Bangkok, 10400, Thailand

    Takuya Nihira

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  1. Ivy Grace Umadhay Pait
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  2. Shigeru Kitani
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Correspondence to Takuya Nihira.

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This research was conducted by I.G.U.P. in partial fulfillment of the requirements for a Ph.D.

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Pait, I.G.U., Kitani, S., Roslan, F.W. et al. Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from Streptomyces lavendulae FRI-5. J Ind Microbiol Biotechnol 45, 77–87 (2018). https://doi.org/10.1007/s10295-017-1997-x

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