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A novel thin-layer chromatography method to screen 1,3-propanediol producers

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Journal of Industrial Microbiology & Biotechnology

Abstract

To date, there is no established protocol for the screening of 1,3-propanediol producers. The proposed method has a wide applicability to harness the commercial potential of microorganisms which produce 1,3-propanediol as the end product. Glycerol fermentation broth of 50 bacteria spotted on thin-layer chromatography plates and run by appropriate solvent systems followed by colour development using vanillin reagent gave different coloured spots with most of the compounds present in the fermentation broth. The appearance of a purple-coloured spot of 1,3-propanediol with a retention factor (R f) of 0.62 forms the basis for the selection of 1,3-propanediol producers. Apart from being a rapid detection system the proposed method is pH independent and its authenticity was reconfirmed by HPLC.

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Acknowledgments

The authors wish to acknowledge the Council of Scientific and Industrial Research (CSIR), Government of India for supporting the study in the form of a grant to Ms. P. Anand who is the recipient of the CSIR-SRF (Grant No. 9/45(949)/2010-EMR-I).

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Authors and Affiliations

  1. Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Pinki Anand & Rajendra Kumar Saxena

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  1. Pinki Anand
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  2. Rajendra Kumar Saxena
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Correspondence to Rajendra Kumar Saxena.

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Anand, P., Saxena, R.K. A novel thin-layer chromatography method to screen 1,3-propanediol producers. J Ind Microbiol Biotechnol 39, 1713–1718 (2012). https://doi.org/10.1007/s10295-012-1161-6

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  • DOI: https://doi.org/10.1007/s10295-012-1161-6

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