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Drug resistance marker-aided genome shuffling to improve acetic acid tolerance in Saccharomyces cerevisiae

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Acetic acid existing in a culture medium is one of the most limiting constraints in yeast growth and viability during ethanol fermentation. To improve acetic acid tolerance in Saccharomyces cerevisiae strains, a drug resistance marker-aided genome shuffling approach with higher screen efficiency of shuffled mutants was developed in this work. Through two rounds of genome shuffling of ultraviolet mutants derived from the original strain 308, we obtained a shuffled strain YZ2, which shows significantly faster growth and higher cell viability under acetic acid stress. Ethanol production of YZ2 (within 60 h) was 21.6% higher than that of 308 when 0.5% (v/v) acetic acid was added to fermentation medium. Membrane integrity, higher in vivo activity of the H+-ATPase, and lower oxidative damage after acetic acid treatment are the possible reasons for the acetic acid-tolerance phenotype of YZ2. These results indicated that this novel genome shuffling approach is powerful to rapidly improve the complex traits of industrial yeast strains.

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Acknowledgments

We thank Dr. Johannes H. Hegemann and Thomas D. Petes for supply of plasmids pSH65 and pFA6a-kanMX4. Thanks also to Henan Tianguan Fuel Ethanol Co. Ltd. for yeast strains providing.

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Authors and Affiliations

  1. Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, People’s Republic of China

    Dao-Qiong Zheng, Xue-Chang Wu, Pin-Mei Wang, Xiao-Qin Chi, Xiang-Lin Tao, Ping Li, Xin-Hang Jiang & Yu-Hua Zhao

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  1. Dao-Qiong Zheng
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  2. Xue-Chang Wu
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Correspondence to Xue-Chang Wu.

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D.-Q. Zheng and X.-C. Wu contributed equally to this work.

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Zheng, DQ., Wu, XC., Wang, PM. et al. Drug resistance marker-aided genome shuffling to improve acetic acid tolerance in Saccharomyces cerevisiae . J Ind Microbiol Biotechnol 38, 415–422 (2011). https://doi.org/10.1007/s10295-010-0784-8

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  • DOI: https://doi.org/10.1007/s10295-010-0784-8

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