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Mannosylerythritol lipids: a review

  • Review
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Mannosylerythritol lipids (MELs) are surface active compounds that belong to the glycolipid class of biosurfactants (BSs). MELs are produced by Pseudozyma sp. as a major component while Ustilago sp. produces them as a minor component. Although MELs have been known for over five decades, they recently regained attention due to their environmental compatibility, mild production conditions, structural diversity, self-assembling properties and versatile biochemical functions. In this review, the MEL producing microorganisms, the production conditions, their applications, their diverse structures and self-assembling properties are discussed. The biosynthetic pathways and the regulatory mechanisms involved in the production of MEL are also explained here.

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References

  1. Bhattacharjee SS, Haskins RH, Gorin PAJ (1970) Location of acyl groups on two partly acylated glycolipids from strains of Ustilago (smut fungi). Carbohydr Res 13:235–246. doi:10.1016/S0008-6215(00)80830-7

    Article  CAS  Google Scholar 

  2. Boothroyd B, Thorn JA, Haskins RH (1956) Biochemistry of the ustilaginales. XII. Characterization of extracellular glycolipids produced by Ustilago sp. Can J Biochem Physiol 34:10–14

    PubMed  CAS  Google Scholar 

  3. Cameotra SS, Makkar RS (2004) Recent applications of biosurfactants as biological and immunological molecules. Curr Opin Microbiol 7:262–266. doi:10.1016/j.mib.2004.04.006

    Article  PubMed  CAS  Google Scholar 

  4. Davey ME, Caiazza NC, OToole GA (2003) Rhamnolipid surfactant production affects biofilm architecture in Pseudomonas aeruginosa PAO1. J Bacteriol 185:1027–1036. doi:10.1128/JB.185.3.1027-1036.2003

    Article  PubMed  CAS  Google Scholar 

  5. Deml G, Anke T, Oberwinkler F, Gianetti BM, Steglich W (1980) Schizonellin A and B, new glycolipids from Schizonella melanogramma. Phytochemistry 19:83–87. doi:10.1016/0031-9422(80)85018-7

    Article  CAS  Google Scholar 

  6. Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61:47–64

    PubMed  CAS  Google Scholar 

  7. Deziel E, Lepine F, Milot S, Villemur R (2003) rhlA is required for the production of a novel biosurfactant promoting swarming motility in Pseudomonas aeruginosa: 3-(3-hydroyalkanoyloxy)alkanoic acids (HAAs), the precursors of rhamnolipids. Microbiology 149:2005–2013. doi:10.1099/mic.0.26154-0

    Article  PubMed  CAS  Google Scholar 

  8. Fluharty AL, O’Brien JS (1969) A mannose- and erythritol-containing glycolipid from Ustilago maydis. Biochemistry 8:2627–2632. doi:10.1021/bi00834a056

    Article  PubMed  CAS  Google Scholar 

  9. Fukuoka T, Morita T, Konishi M, Imura T, Kitamoto D (2007) Characterization of new glycolipid biosurfactants, tri-acylated mannosylerythritol lipids, produced by Pseudozyma yeasts. Biotechnol Lett 29:1111–1118. doi:10.1007/s10529-007-9363-0

    Article  PubMed  CAS  Google Scholar 

  10. Fukuoka T, Morita T, Konishi M, Imura T, Kitamoto D (2007) Characterisation of new types of mannosylerythritol lipids as biosurfactants produced from soybean oil by a Basidiomycetes yeast, Pseudozyma shanxiensis. J Oleo Sci 56:435–442

    PubMed  CAS  Google Scholar 

  11. Fukuoka T, Morita T, Konishi M, Imura T, Sakai H, Kitamoto D (2007) Structural characterization and surface-active properties of a new glycolipid biosurfactant, mono-acylated mannosylerythritol lipid, produced from glucose by Pseudozyma antarctica. Appl Microbiol Biotechnol 76:801–810. doi:10.1007/s00253-007-1051-4

    Article  PubMed  CAS  Google Scholar 

  12. Fukuoka T, Morita T, Konishi M, Imura T, Kitamoto D (2008) A basidiomycetous yeast, Pseudozyma tsukubaensis, efficiently produces a novel glycolipid biosurfactant. The identification of a new diastereomer of mannosylerythritol lipid-B. Carbohydr Res 343:555–560

    Article  PubMed  CAS  Google Scholar 

  13. Hamme JDV, Singh A, Ward OP (2006) Physiological aspects Part 1 in a series of papers devoted to surfactants in microbiology and biotechnology. Biotechnol Adv 24:604–620. doi:10.1016/j.biotechadv.2006.08.001

    Article  PubMed  CAS  Google Scholar 

  14. Haskins RH, Thorn JA, Boothroyd B (1955) Biochemistry of the ustilagenales. XI. Metabolic products of Ustilago zeae in submerged culture. Can J Microbiol 1:749–756

    Article  PubMed  CAS  Google Scholar 

  15. Im JH, Nakane T, Yanagishita H, Ikegami T, Kitamoto D (2001) Mannosylerythritol lipid, a yeast extracellular glycolipid, shows high binding affinity towards human immunoglobulin G. BMC Biotechnol 1:5. http://www.biomedcentral.com/1472-6750/1/5. doi:10.1186/1472-6750-1-5

  16. Im JH, Yanagishita H, Ikegami T, Takeyama Y, Idemoto Y, Koura N et al (2003) Mannosylerythritol lipids, yeast glycolipid biosurfactants, are potential affinity ligand materials for human immunoglobulin G. J Biomed Mater Res 65A:379–385. doi:10.1002/jbm.a.10491

    Article  CAS  Google Scholar 

  17. Imura T, Yanagishita H, Kitamoto D (2004) Coacervate formation from natural glycolipid: one acetyl group on the headgroup triggers coacervate-to-vesicle transition. J Am Chem Soc 126:10804–10805. doi:10.1021/ja0400281

    Article  PubMed  CAS  Google Scholar 

  18. Imura T, Yanagishita H, Ohira J, Sakai H, Abeb M, Kitamoto D (2005) Thermodynamically stable vesicle formation from glycolipid biosurfactant sponge phase. Colloids Surf B Biointerfaces 43:115–121. doi:10.1016/j.colsurfb.2005.03.015

    Article  PubMed  CAS  Google Scholar 

  19. Imura T, Ohta N, Inoue K, Yagi N, Negishi H, Yanagishita H et al (2006) Naturally engineered glycolipid biosurfactants leading to distinctive self-assembled structures. Chem Eur J 12:2434–2440. doi:10.1002/chem.200501199

    Article  CAS  Google Scholar 

  20. Imura T, Hikosaka Y, Worakitkanchanakul W, Sakai H, Abe M, Konishi M et al (2007) Aqueous-phase behavior of natural glycolipid biosurfactant mannosylerythritol lipid A: sponge, cubic, and lamellar phases. Langmuir 23:1659–1663. doi:10.1021/la0620814

    Article  PubMed  CAS  Google Scholar 

  21. Imura T, Ito S, Azumi R, Yanagishita H, Sakai H, Abe M et al (2007) Monolayers assembled from a glycolipid biosurfactant from Pseudozyma (Candida) antarctica serve as a high-affinity ligand system for immunoglobulin G and M. Biotechnol Lett 29:865–870. doi:10.1007/s10529-007-9335-4

    Article  PubMed  CAS  Google Scholar 

  22. Inaba H (2000) New challenge in advanced thermal energy transportation using functionally thermal fluids. Int J Therm Sci 39:991–1003. doi:10.1016/S1290-0729(00)01191-1

    Article  CAS  Google Scholar 

  23. Inoh Y, Kitamoto D, Hirashima N, Nakanishi M (2001) Biosurfactants of MEL-A increase gene transfection mediated by cationic liposomes. Biochem Biophys Res Commun 289:57–61. doi:10.1006/bbrc.2001.5930

    Article  PubMed  CAS  Google Scholar 

  24. Inoh Y, Kitamoto D, Hirashima N, Nakanishi M (2004) Biosurfactant MEL-A dramatically increases gene transfection via membrane fusion. J Control Release 94:423–431. doi:10.1016/j.jconrel.2003.10.020

    Article  PubMed  CAS  Google Scholar 

  25. Isoda H, Kitamoto D, Shinmoto H, Matsumura M, Nakahara T (1997) Microbial extracellular glycolipid induction of differentiation and inhibition of the protein kinase C activity of human promyelocytic leukemia cell line HL60. Biosci Biotechnol Biochem 61:609–614

    PubMed  CAS  Google Scholar 

  26. Isoda H, Shinmoto H, Kitamoto D, Matsumura M, Nakahara T (1997) Differentiation of human promyelocytic leukemia cell line HL60 by microbial extracellular glycolipids. Lipids 32:263–271. doi:10.1007/s11745-997-0033-0

    Article  PubMed  CAS  Google Scholar 

  27. Isoda H, Nakahara T (1997) Mannosylerythritol lipid induces granulocytic differentiation and inhibits the tyrosine phosphorylation of human myelogenous leukemia cell line K562. Cytotechnology 25:191–195. doi:10.1023/A:1007982909932

    Article  CAS  Google Scholar 

  28. Kakugawa K, Tamai M, Imamura K, Miyamoto K, Miyoshi S, Morinaga Y et al (2002) Isolation of yeast Kurtzmanomyces sp. I-11, novel producer of mannosylerythritol lipid. Biosci Biotechnol Biochem 66:188–191. doi:10.1271/bbb.66.188

    Article  PubMed  CAS  Google Scholar 

  29. Kawashima H, Nakahara T, Oogaki M, Tabuchi T (1983) Extracellular production of a mannosylerythritol lipid by a mutant of Candida sp. from n-alkanes and triacylglycerols. J Ferment Technol 61:143–149

    CAS  Google Scholar 

  30. Kearns DB, Losick R (2003) Swarming motility in undomesticated Bacillus subtilis. Mol Microbiol 49:581–590. doi:10.1046/j.1365-2958.2003.03584.x

    Article  PubMed  CAS  Google Scholar 

  31. Kim H-S, Yoon BD, Choung DH, Oh HM, Katsuragi T, Tani Y (1999) Characterization of a biosurfactant, mannosylerythritol lipid produced from Candida sp SY16. Appl Microbiol Biotechnol 52:713–721. doi:10.1007/s002530051583

    Article  PubMed  CAS  Google Scholar 

  32. Kim H-S, Jeon J-W, Kim B-H, Ahn C-Y, Oh H-M, Yoon B-D (2006) Extracellular production of a glycolipid biosurfactant, mannosylerythritol lipid, by Candida sp. SY16 using fed-batch fermentation. Appl Microbiol Biotechnol 70:391–396. doi:10.1007/s00253-005-0092-9

    Article  PubMed  CAS  Google Scholar 

  33. Kitamoto D, Akiba S, Hioki C, Tabuchi T (1990) Extracellular accumulation of mannosylerythritol lipids by a strain of Candida antarctica. Agric Biol Chem 54:31–36

    CAS  Google Scholar 

  34. Kitamoto D, Haneishi K, Nakahara T, Tabuchi T (1990) Production of mannosylerythritol lipids by Candida antarctica from vegetable oils. Agric Biol Chem 54:37–40

    CAS  Google Scholar 

  35. Kitamoto D, Fuzishiro T, Yanagishita H, Nakane T, Nakahara T (1992) Production of mannosylerythritol lipids as biosurfactants by resting cells of Candida antarctica. Biotechnol Lett 14:305–310. doi:10.1007/BF01022329

    Article  CAS  Google Scholar 

  36. Kitamoto D, Nemoto T, Yanagishita H, Nakane T, Kitamoto H, Nakahara T (1993) Fatty acid metabolism of mannosylerythritol lipids as biosurfactants produced by Candida antarctica. J Jpn Oil Chem Soc 42:346–358

    CAS  Google Scholar 

  37. Kitamoto D, Yanagishita H, Shinbo T, Nakane T, Kamisawa C, Nakahara T (1993) Surface active properties and antimicrobial activities of mannosylerythritol lipids as biosurfactants produced by Candida antarctica. J Biotechnol 29:91–96. doi:10.1016/0168-1656(93)90042-L

    Article  CAS  Google Scholar 

  38. Kitamoto D, Yanagishita H, Hayara K, Kltamoto HK (1998) Contribution of a chain-shortening pathway to the biosynthesis of the fatty acids of mannosyierythritol lipid (biosurfactant) in the yeast Candida antarctica: effect of β-oxidation inhibitors on biosurfactant synthesis. Biotechnol Lett 20:813–818. doi:10.1023/A:1005347022247

    Article  CAS  Google Scholar 

  39. Kitamoto D, Ghosh SGO, Nakatani Y (2000) Formation of giant vesicle from diacylmannosylerythritols and their binding to concanavalin A. Chem Commun 2000:861–862. doi:10.1039/b000968g

    Article  Google Scholar 

  40. Kitamoto D, Ikegami T, Suzuki GT, Sasaki A, Takeyama Y, Idemoto Y et al (2001) Microbial conversion of n-alkanes into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma (Candida antarctica). Biotechnol Lett 23:1709–1714. doi:10.1023/A:1012464717259

    Article  CAS  Google Scholar 

  41. Kitamoto D, Yanagishita H, Endo A, Nakaiwa M, Nakane M, Akiya T (2001) Remarkable antiagglomeration effect of a yeast biosurfactant, diacylmannosylerythritol, on ice-water slurry for cold thermal storage. Biotechnol Prog 17:362–365. doi:10.1021/bp000159f

    Article  PubMed  CAS  Google Scholar 

  42. Kitamoto D, Isoda H, Nakahara T (2002) Functions and potential applications of glycolipid biosurfactants-from energy-saving materials to gene delivery carriers. J Biosci Bioeng 94:187–201. doi:10.1263/jbb.94.187

    Article  PubMed  CAS  Google Scholar 

  43. Kitamoto D (2008) Naturally engineered glycolipid biosurfactants leading to distinctive self-assembling properties. Yakugaku Zasshi 128:695–706. doi:10.1248/yakushi.128.695

    Article  PubMed  CAS  Google Scholar 

  44. Konishi M, Imura T, Fukuoka T, Morita T, Kitamoto D (2007) A yeast glycolipid biosurfactant, mannosylerythritol lipid, shows high binding affinity towards lectins on a self-assembled monolayer system. Biotechnol Lett 29:473–480. doi:10.1007/s10529-006-9261-x

    Article  PubMed  CAS  Google Scholar 

  45. Konishi M, Morita T, Fukuoka T, Imura T, Kakugawa K, Kitamoto D (2007) Production of different types of mannosylerythritol lipids as biosurfactants by the newly isolated yeast strains belonging to the genus Pseudozyma. Appl Microbiol Biotechnol 75:521–531. doi:10.1007/s00253-007-0853-8

    Article  PubMed  CAS  Google Scholar 

  46. Konishi M, Morita T, Fukuoka T, Imura T, Kakugawa K, Kitamoto D (2008) Efficient production of mannosylerythritol lipids with high hydrophilicity by Pseudozyma hubeiensis KM-59. Appl Microbiol Biotechnol 78:37–46. doi:10.1007/s00253-007-1292-2

    Article  PubMed  CAS  Google Scholar 

  47. Matsuyama T, Nakagawa Y (1996) Bacterial wetting agents working incolonization of bacteria on surface environments. Colloids Surf B Biointerfaces 7:207–214. doi:10.1016/0927-7765(96)01300-8

    Article  CAS  Google Scholar 

  48. Morita T, Konishi M, Fukuoka T, Imura T, Kitamoto D (2006) Discovery of Pseudozyma rugulosa NBRC 10877 as a novel producer of the glycolipid biosurfactants, mannosylerythritol lipids, based on rDNA sequence. Appl Microbiol Biotechnol 73:305–313. doi:10.1007/s00253-006-0466-7

    Article  PubMed  CAS  Google Scholar 

  49. Morita T, Konishi M, Fukuoka T, Imura T, Kitamoto HK, Kitamoto D (2007) Characterization of the genus Pseudozyma by the formation of glycolipid biosurfactants, mannosylerythritol lipids. FEMS Yeast Res 7:286–292. doi:10.1111/j.1567-1364.2006.00154.x

    Article  PubMed  CAS  Google Scholar 

  50. Morita T, Konishi M, Fukuoka T, Imura T, Yamamoto S, Kitagawa M et al (2008) Identification of Pseudozyma graminicola CBS 10092 as a producer of glycolipid biosurfactants, mannosylerythritol lipids. J Oleo Sci 57:123–131

    PubMed  CAS  Google Scholar 

  51. Morita T, Konishi M, Fukuoka T, Imura T, Kitamoto D (2008) Production of glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma siamensis CBS 9960 and their interfacial properties. J Biosci Bioeng 105:493–502. doi:10.1263/jbb.105.493

    Article  PubMed  CAS  Google Scholar 

  52. Mulligan CN (2005) Environmental applications for biosurfactants. Environ Pollut 133:183–198. doi:10.1016/j.envpol.2004.06.009

    Article  PubMed  CAS  Google Scholar 

  53. Neu TR (1996) Significance of bacterial surface-active compounds in interaction of bacteria with interfaces. Microbiol Rev 60:151–166

    PubMed  CAS  Google Scholar 

  54. Rau U, Nguyen LA, Schulz S, Wray V, Nimtz M, Roeper H et al (2005) Formation and analysis of mannosylerythritol lipids secreted by Pseudozyma aphidis. Appl Microbiol Biotechnol 66:551–5593. doi:10.1007/s00253-004-1672-9

    Article  PubMed  CAS  Google Scholar 

  55. Rau U, Nguyen LA, Roeper H, Koch H, Lang S (2005) Fed-batch bioreactor production of mannosylerythritol lipids secreted by Pseudozyma aphidis. Appl Microbiol Biotechnol 68:607–613. doi:10.1007/s00253-005-1906-5

    Article  PubMed  CAS  Google Scholar 

  56. Rau U, Nguyen LA, Roeper H, Koch H, Lang S (2005) Downstream processing of mannosylerythritol lipids produced by Pseudozyma aphidis. Eur J Lipid Sci Technol 107:373–380. doi:10.1002/ejlt.200401122

    Article  CAS  Google Scholar 

  57. Rodrigues L, Banat IM, Teixeira J, Oliveira R (2006) Biosurfactants: potential applications in medicine. J Antimicrob Chemother 57:609–618. doi:10.1093/jac/dkl024

    Article  PubMed  CAS  Google Scholar 

  58. Ron EZ, Rosenberg E (2001) Natural roles of biosurfactants. Environ Microbiol 3:229–236. doi:10.1046/j.1462-2920.2001.00190.x

    Article  PubMed  CAS  Google Scholar 

  59. Rosenberg E, Ron EZ (1999) High- and low-molecular-mass microbial surfactants. Appl Microbiol Biotechnol 52:154–162. doi:10.1007/s002530051502

    Article  PubMed  CAS  Google Scholar 

  60. Shibahara M, Zhao X, Wakamatsu Y, Nomura N, Nakahara T, Jin C et al (2000) Mannosylerythritol lipid increases levels of galactoceramide in and neurite outgrowth from PC12 pheochromocytoma cells. Cytotechnology 33:247–251. doi:10.1023/A:1008155111024

    Article  CAS  PubMed  Google Scholar 

  61. Singh A, Van Hamme JD, Ward OP (2007) Surfactants in microbiology and biotechnology. Part 2. Application aspects. Biotechnol Adv 25:99–121. doi:10.1016/j.biotechadv.2006.10.004

    Article  PubMed  CAS  Google Scholar 

  62. Singh P, Cameotra SS (2004) Potential applications of microbial surfactants in biomedical sciences. Trends Biotechnol 22:142–146. doi:10.1016/j.tibtech.2004.01.010

    Article  PubMed  CAS  Google Scholar 

  63. Spoeckner S, Wray V, Nimtz M, Lang S (1999) Glycolipids of the smut fungus Ustilago maydis from cultivation on renewable resources. Appl Microbiol Biotechnol 51:33–39. doi:10.1007/s002530051359

    Article  CAS  Google Scholar 

  64. Tanaka A, Fukui S (1989) Metabolism of n-alkane. In: The yeast, vol 3, 2nd edn. Academic Press, London, p 261–287

  65. Ueno Y, Hirashima N, Inoh Y, Furuno T, Nakanishi M (2007) Characterization of biosurfactant-containing liposomes and their efficiency for gene transfection. Biol Pharm Bull 30:169–172. doi:10.1248/bpb.30.169

    Article  PubMed  CAS  Google Scholar 

  66. Ueno Y, Inoh Y, Furuno T, Hirashima N, Kitamoto D, Nakanishi M (2007) NBD-conjugated biosurfactant (MEL-A) shows a new pathway for transfection. J Control Release 123:247–253. doi:10.1016/j.jconrel.2007.08.012

    Article  PubMed  CAS  Google Scholar 

  67. Vertesy L, Kurz M, Wink J, Noelken G (2002) Ustilipides, method for the production and the use thereof. US Patent 6,472,158

  68. Wakamatsu Y, Zhao X, Jin C, Day N, Shibahara M, Nomura N et al (2001) Mannosylerythritol lipid induces characteristics of neuronal differentiation in PC12 cells through an ERK-related signal cascade. Eur J Biochem 268:374–383. doi:10.1046/j.1432-1033.2001.01887.x

    Article  PubMed  CAS  Google Scholar 

  69. Wander RJA, Vreken P, Ferdiandusse S, Jansen GA, Waterham HR, van Roermunde CWT et al (2001) Peroxisomal fatty acid α- and β-oxida tion in humans: enzymology, peroxisomal metabolite transporters and peroxisomal diseases. Biochem Soc Trans 29:250–267. doi:10.1042/BST0290250

    Article  Google Scholar 

  70. Worakitkanchanakula W, Imurab T, Fukuokab T, Moritab T, Sakaia H, Abea M et al. (2008) Aqueous-phase behavior and vesicle formation of natural glycolipid biosurfactant, mannosylerythritol lipid-B. Colloids Surf B Biointerfaces. doi:10.1016/j.colsurfb.2008.03.009

  71. Zhao X, Wakamatsu Y, Shibahara M, Nomura N, Geltinger C, Nakahara T et al (1999) Mannosylerythritol lipid is a potent inducer of apoptosis and differentiation of mouse melanoma cells in culture. Cancer Res 59:482–486

    PubMed  CAS  Google Scholar 

  72. Zhao X, Geltinger X, Kishikawa S, Ohshima S, Murata S, Nomura S et al (2000) Treatment of mouse melanoma cells with phorbol 12-myristate 13-acetate counteracts mannosylerythritollipid-induced growth arrest and apoptosis. Cytotechnology 33:123–130. doi:10.1023/A:1008129616127

    Article  CAS  PubMed  Google Scholar 

  73. Zhao X, Murata T, Ohno S, Day N, Song J, Nomura N et al (2001) Protein kinase Cα plays a critical role in mannosylerythritol lipid-induced differentiation of melanoma B16 Cells. J Biol Chem 276:39903–39910. doi:10.1074/jbc.M010281200

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Madras, Chennai, 36, India

    Joseph Irudayaraj Arutchelvi & Mukesh Doble

  2. Polymer Research and Technology Center, Reliance Industries Limited, Chembur, Mumbai, 71, India

    Sumit Bhaduri & Parasu Veera Uppara

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Arutchelvi, J.I., Bhaduri, S., Uppara, P.V. et al. Mannosylerythritol lipids: a review. J Ind Microbiol Biotechnol 35, 1559–1570 (2008). https://doi.org/10.1007/s10295-008-0460-4

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