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Whole cell microbial transformation in cloud point system

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Journal of Industrial Microbiology & Biotechnology

Abstract

Cloud point system, consisting of nonionic surfactant in an aqueous solution, has been developed as a novel medium for whole cell microbial transformation. The basic properties of cloud point system including phase separation and solubilization are introduced. The application of cloud point system for extractive microbial transformation is different from that of water-organic solvent two-phase partitioning system or aqueous two-phase system are discussed, which mainly focus on the biocompatibility of microorganism in a cloud point system and a downstream process of microbial transformation in cloud point system with oil-water-surfactant microemulsion liquid-liquid extraction for surfactant recovery and product separation. Finally, examples of whole cell microbial transformation in cloud point systems, especially in situ extraction of moderate polar substrate/product, are also presented.

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Acknowledgment

The project was financially supported by the National Natural Science Foundation of China (No. 20676080) and the Science and Technology Expert Foundation of Lily Magnolia, Shanghai, China (No.2007B062).

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Authors and Affiliations

  1. School of Pharmacy, Shanghai Jiao Tong University, 200240, Shanghai, People’s Republic of China

    Zhilong Wang

  2. Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People’s Republic of China

    Jian-He Xu

  3. Shanghai Institutes of Pharmacy Industry, 200040, Shanghai, People’s Republic of China

    Daijie Chen

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  1. Zhilong Wang
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  2. Jian-He Xu
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  3. Daijie Chen
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Correspondence to Zhilong Wang.

Additional information

The similar abstract had been submitted for oral lecture of the 2007 SIM annual meeting at the Hyatt Regency, Denver, USA, from July 29 to August 2.

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Wang, Z., Xu, JH. & Chen, D. Whole cell microbial transformation in cloud point system. J Ind Microbiol Biotechnol 35, 645–656 (2008). https://doi.org/10.1007/s10295-008-0345-6

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