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Growth temperature and OprF porin affect cell surface physicochemical properties and adhesive capacities of Pseudomonas fluorescens MF37

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Pseudomonads adapt to various ecological niches by forming biofilms, which first requires bacterial adhesion on surfaces. We studied the influence of growth temperature on surface physicochemical properties of Pseudomonas fluorescens MF37 and on its adhesive capacities onto inert surfaces. It presented a global hydrophilic character, measured by microbial adhesion to solvent (MATS), and showed a cell surface more hydrophilic at 8 and 28°C than at 17°C. Moreover, P. fluorescens MF37 was more adhesive at 17°C. This critical temperature thus should be carefully taken into account in food safety. Adhesion onto inert surfaces is thus influenced by the growth temperature, which modifies the bacteria cell wall properties through changes in the outer membrane components. Therefore, we studied the effect of the loss of OprF, the major outer membrane protein, known to act as an adhesin (root, and endothelial cells). The OprF-deficient mutant was able to adhere to surfaces, but showed the same physicochemical and adhesion properties on abiotic surfaces whatever the growth temperature. OprF is thus not essential in this adhesion process. However, we suggest that OprF is involved in the bacterial environmental temperature sensing by P. fluorescens.

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Authors and Affiliations

  1. Laboratoire de Microbiologie Du Froid, EA 2123, Université de Rouen, 55 rue St Germain, 27000, Evreux, France

    Gaëlle Hemery, Sylvie Chevalier & Nicole Orange

  2. Laboratoire de Biotechnologie et Chimie Marines, EA 3884, Université de Bretagne Sud, rue St Maudé, 56321, Lorient, France

    Gaëlle Hemery & Dominique Haras

  3. Unité de Recherche en Bioadhésion et Hygiène des Matériaux, INRA, 25 rue de la république, 91744, Massy, France

    Gaëlle Hemery & Marie-Noëlle Bellon-Fontaine

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  1. Gaëlle Hemery
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  2. Sylvie Chevalier
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  3. Marie-Noëlle Bellon-Fontaine
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  4. Dominique Haras
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  5. Nicole Orange
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Correspondence to Nicole Orange.

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This work was supported by a grant from the Région Bretagne (Doctoral fellowship to G.H.).

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Hemery, G., Chevalier, S., Bellon-Fontaine, MN. et al. Growth temperature and OprF porin affect cell surface physicochemical properties and adhesive capacities of Pseudomonas fluorescens MF37. J Ind Microbiol Biotechnol 34, 49–54 (2007). https://doi.org/10.1007/s10295-006-0160-x

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  • DOI: https://doi.org/10.1007/s10295-006-0160-x

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