Summary
The gene encoding the root adhesin from the outer membrane of Pseudomonas fluorescens OE 28.3 was isolated from a genomic λEMBL3 library and sequenced. The deduced protein (32104 daltons) displayed strong homology with the amino- and carboxyterminal parts of porin F (OprF) from P. aeruginosa and P. syringae. Significant homology was also found within the C-terminal domain of the OmpA proteins from Enterobacteria and major outer membrane proteins from Neisseria species. However, a cysteine-rich domain present in the OprFs of P. aeruginosa and P. syringae is absent from the adhesin of P. fluorescens. Instead, it contains a shorter sequence with eight alternating proline residues.
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Communicated by J. Schell
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De Mot, R., Proost, P., Van Damme, J. et al. Homology of the root adhesin of Pseudomonas fluorescens OE 28.3 with porin F of P. neruginosa and P. syringae . Molec. Gen. Genet. 231, 489–493 (1992). https://doi.org/10.1007/BF00292721
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DOI: https://doi.org/10.1007/BF00292721