Abstract
The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0.72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.
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Acknowledgments
This work was supported by grants from DICYT-USACH, FONDECYT 1100084 and UNAB DI-03-10/R. I. Vaca acknowledges support from the “Programa Bicentenario de Ciencia y Tecnología” program (Chile), project PDA13. Technical assistance of Carlos Aguirre is gratefully acknowledged.
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Ravanal, MC., Espinosa, Y., Rosa, L. et al. Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans . Mycoscience 53, 152–155 (2012). https://doi.org/10.1007/s10267-011-0144-1
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DOI: https://doi.org/10.1007/s10267-011-0144-1