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Culture conditions for enhanced cellulase production by a native strain of Penicillium purpurogenum

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Abstract

A cellulolytic wild-type strain of Penicillium purpurogenum was isolated from a soil sample in southern Chile. It grew best at 28°C from an inoculum of 4×107 spores/100 ml medium. Highest endoglucanase activity was with Sigmacell as carbon source and corn steep liquor as nitrogen source. Wheat bran enhanced the production of endoglucanase and β-glucosidase. The enzymes in the crude supernatants were stable up to 50°C and between pH 4.4 and 5.6 for 48 h.

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Authors
  1. J. Steiner
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  2. C. Socha
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  3. J. Eyzaguirre
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Additional information

J.Steiner and C. Socha are with the Facultad de Ciencias Quimicas y Farmacéuticas, Universidad de Chile, Casilla 233, Santiago 1, Chile; J. Eyzaguirre is with the Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.

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Steiner, J., Socha, C. & Eyzaguirre, J. Culture conditions for enhanced cellulase production by a native strain of Penicillium purpurogenum . World Journal of Microbiology & Biotechnology 10, 280–284 (1994). https://doi.org/10.1007/BF00414863

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  • DOI: https://doi.org/10.1007/BF00414863

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