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Physicochemical and microbiological assessment of a dental adhesive doped with cashew nut shell liquid

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Abstract

To evaluate i) the inhibitory and bactericidal activity of cashew nut shell liquid (CNSL) and its isolated compounds (anacardic acid and cardol) against oral bacteria; ii) the biofilm formation inhibition, resin-dentin bond strength and physicochemical properties of a dental adhesive incorporated with these substances. The antibacterial effect of CNSL, anacardic acid, and cardol were assessed by determining the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations. Effect in inhibiting biofilm formation of the adhesive incorporated with the substances (15 μg/ml) against a mixed-species biofilm of Streptococcus mutans and Candida Albicans and was determined by direct contact test. Additional Analysis included microtensile bond strength (μTBS) test, elastic modulus (EM), flexural strength (FS), degree of conversion (DC), water sorption (WS) and solubility (SL). The data were submitted to statistical analysis by one-way ANOVA and Tukey’s test (p < 0.05). CNSL, anacardic acid and cardol showed antibacterial activity for all strains tested, with MIC and MBC values ranging from 3.12 to 25 μg/ml. There was no growth of colonies forming units in the adhesives incorporated with the substances. EM increased in the adhesive incorporated with anacardic acid, decreased after incorporation of cardol and it was not affected by incorporation of CNSL. The substances tested showed no effect in FS, DC, WS, SL and μTBS. In conclusion, the CNSL, anacardic acid and cardol showed antibacterial effects against oral bacteria and, the incorporation of substances did not reduce the performance of the adhesive.

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Acknowledgements

This work was financially supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [grant numbers 88882.454131/2019-01]. The authors would like to thank the generous donations of adhesive system Ambar APS by FGM.

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Authors and Affiliations

  1. Department of Restorative Dentistry, Federal University of Ceará, 949 Alexandre Baraúna St., Fortaleza, Ceará, 60430-160, Brazil

    Nayara de Oliveira Souza, Diana Araújo Cunha, Nara Sousa Rodrigues & Vicente de Paulo Aragão Saboia

  2. Department of Organic and Inorganic Chemistry, Federal University of Ceará, 100 Five St., Fortaleza, Ceará, 60355-636, Brazil

    Thayllan Teixeira Bezerra, Diego Lomonaco & Selma Elaine Mazzetto

  3. Research Laboratory of Applied Microbiology, University of Franca, 201 Dr. Armando de Sáles Oliveira Ave., Franca, São Paulo, 14404-600, Brazil

    Carlos Henrique Gomes Martins & Luciana Assirati Casemiro

  4. Olímpio Galdino de Sousa 465/1302 Street, W/N, Guararapes, Fortaleza, Ceará, 60810-005, Brazil

    Vicente de Paulo Aragão Saboia

Authors
  1. Nayara de Oliveira Souza
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  2. Diana Araújo Cunha
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  8. Luciana Assirati Casemiro
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  9. Vicente de Paulo Aragão Saboia
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Contributions

NdOS: investigation, validation, writing—original draft. DAC: writing—original draft, conceptualization. NdSR: writing—original draft, formal analysis. TTB: investigation. DL: methodology, resources, writing—review and editing. SEM: methodology, investigation. CHGM: visualization, methodology. LAC: resources, writing—review and editing. VdPAS: supervision, project administration, writing—review and editing.

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Correspondence to Vicente de Paulo Aragão Saboia.

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de Oliveira Souza, N., Cunha, D.A., Sousa Rodrigues, N. et al. Physicochemical and microbiological assessment of a dental adhesive doped with cashew nut shell liquid. Odontology 110, 434–443 (2022). https://doi.org/10.1007/s10266-021-00672-7

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  • DOI: https://doi.org/10.1007/s10266-021-00672-7

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