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Antimicrobial efficacy and mechanical properties of BAC-modified hard and soft denture liners

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Abstract

This study investigated the antimicrobial efficacy and mechanical strength of hard and soft denture liners modified with benzalkonium chloride (BAC). The specimens (1 mm thickness, 8 mm diameter) were prepared by mixing 0.5, 1, 2 and 5 wt% BAC with soft (Sofreliner Medium, Tokuyama) and hard (Rebase II, Tokuyama) denture liners (n = 5/group). BAC was not added to the controls. Candida albicans ATCC 28366 (A 550 = 0.5) and Streptococcus mutans Ingbritt suspensions (A 550 = 0.35) were pipetted onto the specimens, and incubated for 4 h. The viable cells were collected, and determined by plate-culturing (CFU). The tests were repeated after the specimens were soaked in distilled water for 7 days. The mechanical strengths were evaluated by tear and 4-point flexural strength tests for soft and hard liners, respectively. The data were analyzed with ANOVA and Tukey’s HSD tests at p = 0.05. C. albicans viability was lost in all groups of BAC-modified soft liners (p < 0.001), and S. mutans viability was reduced (p < 0.01), except of soaked BAC 0.5 wt% group (p > 0.05). For the hard liner, BAC 5 wt% killed the C. albicans and S. mutans cells both before and after soaked in water (p < 0.001). BAC 2 wt% showed comparable tear strength with the soft liner control (p > 0.05). BAC did not reduce the flexural strength of the hard liner (p > 0.05), except of BAC 5 wt% group (p < 0.01). BAC can be a promising agent reducing the C. albicans and S. mutans viability on the soft and hard denture liner surfaces.

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Acknowledgements

This study was supported by Grant #296653 from the Academy of Finland to AT-M (PI), EVO funding of Turku University Hospital to AT-M (PI). The authors do not have a financial interest in products, equipment, and companies cited in the manuscript. The authors also would like to thank to the biomedical laboratory technician Oona Hällfors for performing antimicrobial tests, and to Kaveh Nik Jamal, M.Sc for his assistance during the mechanical testing procedures in this study. The soft and hard denture liners used in this study were generously donated by Tokuyama Corp., Japan.

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Authors and Affiliations

  1. Finnish Doctoral Program in Oral Sciences, Turku, Finland

    Pinar Altinci

  2. Department of Restorative Dentistry and Cariology, Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520, Turku, Finland

    Pinar Altinci, Murat Mutluay, Eva Söderling & Arzu Tezvergil-Mutluay

  3. TYKS University Hospital, University of Turku, Turku, Finland

    Murat Mutluay & Arzu Tezvergil-Mutluay

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  1. Pinar Altinci
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  2. Murat Mutluay
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  4. Arzu Tezvergil-Mutluay
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Correspondence to Pinar Altinci.

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Altinci, P., Mutluay, M., Söderling, E. et al. Antimicrobial efficacy and mechanical properties of BAC-modified hard and soft denture liners. Odontology 106, 83–89 (2018). https://doi.org/10.1007/s10266-017-0303-8

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  • DOI: https://doi.org/10.1007/s10266-017-0303-8

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