Cell cycle inhibitors improve seed storability after priming treatments
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Seed priming is a treatment that controls seed water content to partially activate germination processes such as metabolism but prevents full germination of the seeds. The treatment is well known to enhance seed performance, including germination, but sometimes reduces seed storability or longevity as a side effect. Toward developing a novel priming technique that can maintain seed longevity for a longer time period, chemicals that suppress the seed deterioration under a controlled condition were screened from 80 known biologically active compounds contained in the RIKEN NPDepo authentic library using Arabidopsis thaliana seeds. Seeds primed with mimosine, a cell cycle inhibitor, retained higher survival rate after a controlled deterioration treatment compared to seeds primed without the chemical. In addition, other cell cycle inhibitors such as aphidicolin, hydroxyurea and oryzalin had similar effects on the seed storability after priming. Our results suggest that progression of the cell cycle during priming is an important checkpoint that determines the storability of seeds after the treatment.
KeywordsCell cycle Chemical screening Longevity Priming Seed desiccation Seed germination
Controlled deterioration treatment
This study was supported by RIKEN Special Postdoctoral Researcher Program to N.S. We thank Dr. Hiroyuki Osada and Dr. Hiroyuki Hirano (RIKEN CSRS) for providing the NPDepo authentic library, the Arabidopsis Biological Resource Center (ABRC) for providing natural accessions, and Ms. Masako Tanaka (RIKEN CSRS) for her assistance with plant growth and seed harvesting.
NS and MS designed the experiments. NS performed the experiments. NS and MS discussed the results and wrote the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Aravind J, Vimala Devi S, Radhamani J, Jacob SR, Srinivasan K (2018) Germination metrics: seed germination indices and curve fitting. R package version 0.1.1. https://cran.r-project.org/package=germinationmetrics, https://doi.org/10.5281/zenodo.1219630. Accessed 20 Sept 2018
- Bino RJ, Vries JD, Kraak HL, Pijlen JV (1992) Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination. Ann Bot 69:231–236. https://doi.org/10.1093/oxfordjournals.aob.a088335 CrossRefGoogle Scholar
- Macovei A, Pagano A, Leonetti P, Carbonera D, Balestrazzi A, Araújo SS (2017) Systems biology and genome-wide approaches to unveil the molecular players involved in the pre-germinative metabolism: implications on seed technology traits. Plant Cell Rep 36:669–688. https://doi.org/10.1007/s00299-016-2060-5 CrossRefGoogle Scholar
- Masubelele NH, Dewitte W, Menges M, Maughan S, Collins C, Huntley R, Nieuwland J, Scofield S, Murray JA (2005) D-type cyclins activate division in the root apex to promote seed germination in Arabidopsis. Proc Natl Acad Sci USA 102:15694–15699. https://doi.org/10.1073/pnas.0507581102 CrossRefGoogle Scholar
- McDonald MB (2000) Seed priming. In: Black M, Bewley JD (eds) Seed technology and its biological basis. Sheffield Academic Press, Sheffield, pp 287–325Google Scholar
- R Core Team (2016) R: a language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
- Raz V, Bergervoet JHV, Koornneef M (2001) Sequential steps for developmental arrest in Arabidopsis seeds. Development 128:243–252Google Scholar
- Selmar D (1999) Biosynthesis of cyanogenic glycosides, glucosinolates and nonprotein amino acids. In: Wink M (ed) Biochemistry of plant secondary metabolism. Sheffield Academic Press, Sheffield, pp 77–150Google Scholar
- Sliwinska E (2000) Analysis of the cell cycle in sugarbeet seed during development, maturation and germination. In: Black M, Bradford KJ, Vazquez-Ramos J (eds) Seed biology: advances and applications. CAB International, Wallingford, pp 133–139Google Scholar
- Varierl A, Vari AK, Dadlani M (2010) The subcellular basis of seed priming. Curr Sci 99:450–456Google Scholar