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Electron transport activities of Arabidopsis thaliana mutants with impaired chloroplastic NAD(P)H dehydrogenase

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Abstract

The activities of electron transport are compared between wild-type Arabidopsis and two Arabidopsis mutants deficient for the chloroplastic NAD(P)H dehydrogenase (NDH) which catalyzes cyclic electron transport around photosystem I. The quantum yield of photosystem II and the degree of non-photochemical quenching of chlorophyll fluorescence were of similar levels in the two NDH-deficient mutants and the wild type under non-stressed standard growth conditions. Stromal over-reduction was induced in Arabidopsis NDH mutants with high light treatment, as is the case in tobacco NDH mutants. However, unlike tobacco mutants, photoinhibition was not observed in the Arabidopsis NDH mutants.

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Fig. 1

Abbreviations

FQR:

Ferredoxin quinone reductase

ΦII :

Photochemical quantum yield of photosystem II

NDH:

NAD(P)H dehydrogenase

NPQ:

Non-photochemical quenching of chlorophyll fluorescence

qP:

Photochemical quenching of chlorophyll fluorescence

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Acknowledgments

We thank the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis T-DNA insertion mutants and also the ABRC for providing seeds of T-DNA insertion mutants. We thank Dr T. Shikanai of Kyoto University for providing seeds of Columbia-gl1 and the pgr5 mutant. This study was supported in part by a Grant-in-Aid for basic research C 19580105 (to TE) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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  1. Department of Plant Genes and Totipotency, Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan

    Noriko Ishikawa, Tsuyoshi Endo & Fumihiko Sato

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  1. Noriko Ishikawa
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  2. Tsuyoshi Endo
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  3. Fumihiko Sato
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Correspondence to Tsuyoshi Endo.

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Ishikawa, N., Endo, T. & Sato, F. Electron transport activities of Arabidopsis thaliana mutants with impaired chloroplastic NAD(P)H dehydrogenase. J Plant Res 121, 521–526 (2008). https://doi.org/10.1007/s10265-008-0180-x

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  • DOI: https://doi.org/10.1007/s10265-008-0180-x

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