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Antineutrophil cytoplasmic antibodies against myeloperoxidase, proteinase 3, elastase, cathepsin G and lactoferrin in Japanese patients with rheumatoid arthritis

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Modern Rheumatology

Abstract

Antineutrophil cytoplasmic antibodies (ANCAs) against myeloperoxidase (MPO), proteinase 3 (PR-3), lactoferrin (LF), cathepsin G (CG) and elastase (EL) were determined to investigate whether the presence of ANCAs is closely related to extra-articular manifestations in Japanese patients with rheumatoid arthritis (RA). Antibodies against MPO, PR-3, LF, CG and EL were determined in sera from 125 patients with RA and 83 sera from patients with other rheumatic diseases by enzyme-linked immunosorbent assay. Clinical manifestations and laboratory parameters of the patients were studied from medical records. Thirty of the 125 (24.0%) RA patients were positive for ANCAs for at least one of these 5 ANCA antigens. Among the 5 ANCAs, anti-LF antibody (anti-LF) (16.8%) was most commonly observed in patients with RA. A higher joint score (JS) and an elevated ESR were demonstrated in ANCA-positive RA patients compared to those of ANCA-negative patients (40.8 ± 43.3, 24.3 ± 26.2, p < 0.05, 44.4 ± 22.4, 28.9 ± 23.6, p < 0.05, respectively). No statistical differences in the presence of interstitial pneumonia, cutaneous vasculitis, rheumatoid nodules and mononeuropathy multiplex were observed between ANCA-positive and ANCA-negative patients. The presence of anti-LF is expected to be of pathological relevance, as the action of anti-LF towards LF results in the inhibition of the anti-inflammatory activity of LF.

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Acknowledgments

This study was supported by grants from the Research Committee on Intractable Vasculitis, the Ministry of Health, Labor and Welfare of Japan, in 1997, and partially funded by the Research Group on Immunomodulatory Therapy for Vasculitis with Synthetic IVIG, the Ministry of Health, Labor and Welfare of Japan, in 2008. It was reported in the Annual Report of the Research Committee of Intractable Vasculitis, the Ministry of Health and Welfare of Japan, 1997, pp 103–109 (in Japanese).

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Author information

Authors and Affiliations

  1. Department of Internal Medicine, Juntendo Koshigaya Hospital, 560 Fukuroyama, Koshigaya, Saitama, 343-0032, Japan

    Issei Kida & Shigeto Kobayashi

  2. Takeuchi Clinic, Shizuoka, Japan

    Ken Takeuchi

  3. Department of Geriatric General Medicine, Juntendo Tokyo Geriatric Medical Center, Tokyo, Japan

    Hiroshi Tsuda

  4. Juntendo University School of Medicine, Tokyo, Japan

    Hiroshi Hashimoto

  5. Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan

    Yoshinari Takasaki

Authors
  1. Issei Kida
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  2. Shigeto Kobayashi
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  3. Ken Takeuchi
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  4. Hiroshi Tsuda
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  5. Hiroshi Hashimoto
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  6. Yoshinari Takasaki
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Corresponding author

Correspondence to Issei Kida.

Appendix

Appendix

See Table 6.

Table 6 Diagnostic criteria for malignant rheumatoid arthritis (MRA)
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Kida, I., Kobayashi, S., Takeuchi, K. et al. Antineutrophil cytoplasmic antibodies against myeloperoxidase, proteinase 3, elastase, cathepsin G and lactoferrin in Japanese patients with rheumatoid arthritis. Mod Rheumatol 21, 43–50 (2011). https://doi.org/10.1007/s10165-010-0356-9

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  • DOI: https://doi.org/10.1007/s10165-010-0356-9

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