Abstract
Background
In order to clarify the interaction between cardiac dysfunction and sodium homeostasis in the kidney, we used a murine model of cardiac dysfunction and investigated the effect on sodium transporters in renal tubular cells.
Methods
Cardiac function was deteriorated by abdominal aortic banding, and the gene expression of sodium transporters in the kidneys was evaluated by real-time RT-PCR and compared with that in the kidneys of control mice.
Results
Gene expression of all three variants of the murine prolactin receptor was enhanced by aortic banding. Upregulated prolactin receptor was distributed in the proximal tubular cells of the pars recta in the deep inner cortex and the outer stripe of the outer medulla. Prolactin has been reported to be a natriuretic hormone that inhibits proximal tubular Na+/K+-ATPase activity, resulting in reduced sodium reabsorption and the acceleration of natriuresis. Inhibition of endogenous prolactin secretion by bromocriptine administration decreased the urine sodium excretion in both aortic banding and control mice. On the other hand, excess exogenous prolactin administration enhanced urine potassium excretion in aortic banding mice. Furthermore, a high-sodium diet accelerated urinary sodium excretion, which was also significantly decreased by inhibition of endogenous prolactin secretion in aortic banding mice.
Conclusion
We reported that the prolactin receptor was upregulated by aortic banding treatment. Prolactin-prolactin receptor interaction in the proximal tubular cells of the pars recta should involve a different mechanism of kaliuresis other than inhibition of Na+/K+-ATPase.
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Acknowledgments
We are grateful for the excellent technical support of N. Imai, K. Yamagiwa, K. Minowa, H. Aita, and T. Watanabe at the Division of Clinical Nephrology and Rheumatology and F. Higuchi at the Division of Applied Molecular Medicine, Niigata University Graduate School of Medical and Dental Sciences. This study was supported by a Grant for the Promotion of Niigata University Research Projects to Y. K.
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All the authors have declared no competing interests.
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Tsuchida, Y., Kaneko, Y., Otsuka, T. et al. Upregulation of prolactin receptor in proximal tubular cells was induced in cardiac dysfunction model mice. Clin Exp Nephrol 18, 65–74 (2014). https://doi.org/10.1007/s10157-013-0820-x
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DOI: https://doi.org/10.1007/s10157-013-0820-x