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Comparative proteomics reveals differential induction of both biotic and abiotic stress response associated proteins in rice during Xanthomonas oryzae pv. oryzae infection

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Abstract

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight disease in rice and brutally affects the yield up to 50 % of total production. Here, we report a comparative proteomics analysis of total foliar protein isolated from infected rice leaves of susceptible Pusa Basmati 1 (PB1) and resistant Oryza longistaminata genotypes. Two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) approaches identified 29 protein spots encoding unique proteins from both the genotypes. Identified proteins belonged to a large number of biological and molecular functions related to biotic and abiotic stress proteins which are potentially involved during Xoo infection. Biotic and abiotic stress-related proteins were induced during Xoo infection, indicating the activation of common stress pathway during bacterial blight infection. Candidate genes conferring tolerance against bacterial blight, which include germin-like protein, putative r40c1, cyclin-dependent kinase C, Ent-isokaur-15-ene synthase and glutathione-dependent dehydroascorbate reductase 1 (GSH-DHAR1), were also induced, with germin-like proteins induced only in the resistant rice genotype O. longistaminata. Energy, metabolism and hypothetical proteins were common among both the genotypes. Further, host defence/stress-related proteins were mostly expressed in resistant genotype O. longistaminata, indicating possible co-evolution of the pathogen and the wild rice, O. longistaminata.

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Abbreviations

2-DE:

Two-dimensional gel electrophoresis

CRD:

Completely randomized design

HR:

Hypersensitive response

IPG:

Immobilized pH gradient

IEF:

Isoelectric focusing

MALDI-TOF:

Matrix-assisted laser desorption/ionization time-of-flight

MS:

Mass spectrometry

PWC:

Pot water holding capacity

SWC:

Soil water capacity

Xoo :

Xanthomonas oryzae pv. oryzae

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Acknowledgments

We thank the financial supports received from the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India under Project No. BT/PR10904/GBD/27/124/2008. The financial support given to the Department of Plant Sciences, University of Hyderabad from the Department of Biotechnology, Govt. of India-funded Centre for Research and Education in Biology and Biotechnology (DBT-CREBB), DST-Fund for Improvement of S&T Infrastructure in Universities and Higher Educational Institutions (DST-FIST, level II support) and University Grants Commission-Special Assistance Programme (UGC-SAP-CAS) are gratefully acknowledged. The authors thank the support received from Directorate of Rice Research, Hyderabad for providing the seed material and Xoo strain DX133. We also thank Prof. A. R. Podile for his critical suggestions and support.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Prof. C. R. Rao Road, Hyderabad, 500046, India

    Anirudh Kumar, Waikhom Bimolata, P. B. Kirti & Irfan Ahmad Ghazi

  2. Proteomics Core Facility, School of Life Sciences, University of Hyderabad, Prof. C. R. Rao Road, Hyderabad, 500046, India

    Monica Kannan

  3. Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Prof. C. R. Rao Road, Hyderabad, 500046, India

    Insaf Ahmed Qureshi

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  1. Anirudh Kumar
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  2. Waikhom Bimolata
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  3. Monica Kannan
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  4. P. B. Kirti
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  5. Insaf Ahmed Qureshi
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  6. Irfan Ahmad Ghazi
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Correspondence to Irfan Ahmad Ghazi.

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Supplementary Table 1

Identification of major differentially expressed foliar proteins from BB-susceptible rice genotype PB1 and highly resistant O. longistaminata during BB infection (DOCX 19 kb).

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Kumar, A., Bimolata, W., Kannan, M. et al. Comparative proteomics reveals differential induction of both biotic and abiotic stress response associated proteins in rice during Xanthomonas oryzae pv. oryzae infection. Funct Integr Genomics 15, 425–437 (2015). https://doi.org/10.1007/s10142-014-0431-y

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  • DOI: https://doi.org/10.1007/s10142-014-0431-y

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