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Characterisation of Single Nucleotide Polymorphisms and Haplotypes of MSTN Associated with Growth Traits in European Sea Bass (Dicentrarchus labrax)

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Abstract

The myostatin (MSTN) gene, known as growth differentiation factor-8 (GDF-8), is a member of the transforming growth factor-β (TGF-β) superfamily and plays a specific inhibitory role during the critical phases of skeletal muscle mass development in vertebrates. This study was conducted to investigate MSTN polymorphisms in harvest size European sea bass reared in Turkey. Nine single nucleotide polymorphisms (SNPs) and two indels were identified in exons 1–3 of MSTN in the European sea bass population The associations between the g.16612A indel located in intron 1 and standard length were significant. The MSTN g.15252 T > A locus in intron 2 was significantly related to the total weight, fillet weight and standard length (P < 0.05). The relationship between the g.14873C > T locus in exon 3 of MSTN and standard height, head length, body length, pre-anal length, abdominal length, post-anal length and head width was significant (P < 0.05). According to the results of the haplotype analysis, two haplogroup and eight haplotype combinations were detected in the population. The haplogroup 2 had significant associations with all measured growth traits (P < 0.05). Thus, SNPs and haplotypes identified in this study could be useful for European sea bass breeding and marker-assisted selection.

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Fig. 1
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Data Availability

The data analyzed in this study are presented in this article and the supplementary information file. The raw data supporting the conclusion can be shared by request.

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Acknowledgements

We are grateful to Ege University Planning and Monitoring Coordination of Organizational Development and Directorate of Library and Documentation for their support in editing and proofreading service of this study.

Funding

This work was supported by the Scientific Research Projects Coordination Unit of Ege University (Project No: FKP-2020–21912).

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Authors and Affiliations

  1. Faculty of Fisheries, Department of Aquaculture, Ege University, İzmir, 35100, Türkiye

    Emel Özcan Gökçek, Bilge Karahan & Kutsal Gamsız

  2. Faculty of Agriculture, Department of Agricultural Biotechnology, Tekirdağ Namık Kemal University, Tekirdağ, 59030, Türkiye

    Raziye Işık

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  1. Emel Özcan Gökçek
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Contributions

E.Ö.G. and R.I. developed the research topic and acted as study principal investigator (PI). E.Ö.G., B.K. and K.G. collected phenotypic data and sampling. E.Ö.G. obtained and analyzed genetic data. E.Ö.G. and R.I. performed statistical analysis and wrote the manuscript. All authors reviewed the manuscript.

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Correspondence to Emel Özcan Gökçek.

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Özcan Gökçek, E., Işık, R., Karahan, B. et al. Characterisation of Single Nucleotide Polymorphisms and Haplotypes of MSTN Associated with Growth Traits in European Sea Bass (Dicentrarchus labrax). Mar Biotechnol 25, 347–357 (2023). https://doi.org/10.1007/s10126-023-10211-w

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