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Identification of Quantitative Trait Loci Associated with the Skeletal Deformity LSK complex in Gilthead Seabream (Sparus aurata L.)

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Abstract

Morphological abnormalities, especially skeletal deformities, are some of the most important problems affecting gilthead seabream (Sparus aurata L.) aquaculture industry. In this study, a QTL analysis for LSK complex deformity in gilthead seabream is reported. LSK complex is a severe deformity consisting of a consecutive repetition of three vertebral deformities: lordosis, scoliosis, and kyphosis. Seventy-eight offspring from six breeders from a mass-spawning were analyzed: five full-sibling families, three maternal, and two paternal half-sibling families. They had shown a significant association with the LSK complex prevalence in a previous segregation analysis. Fish were genotyped using a set of multiplex PCRs (ReMsa1-13), which includes 106 microsatellite markers. Two methods were used to perform the QTL analysis: a linear regression with the GridQTL software and a linear mixed model with the Qxpak software. A total of 18 QTL were identified. Four of them (QTLSK3, 6, 12, and 14), located in LG5, 8, 17, and 20, respectively, were the most solid ones. These QTL were significant at genome level and showed an extremely large effect (>35 %) with both methods. Markers close to the identified QTL showed a strong association with phenotype. Two of these molecular markers (DId-03-T and Bt-14-F) were considered as potential linked-to-this-deformity markers. The detection of these QTL supposes a critical step in the implementation of marker-assisted selection in this species, which could decrease the incidence of this deformity and other related deformities. The identification of these QTL also represents a major step towards the study of the etiology of skeletal deformities in this species.

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Acknowledgments

We are grateful to Guacimara Alejandro, Sara Rodríguez, and Ivonne Lee for their technical support; to Dionisio Lorenzo for his suggestions and to M. Carmen Rodríguez Valdovinos for her help. This study was supported by the “Search of deformities QTL in gilthead seabream” research project (Plan Nacional de I + D + i). Davinia Negrín-Báez was supported by a grant from the Cabildo Insular de Gran Canaria.

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Authors and Affiliations

  1. Grupo de Investigación en Acuicultura (GIA), Universidad de Las Palmas de Gran Canaria (ULPGC), Carretera Trasmontaña s/n, 35413, Arucas, Las Palmas, Spain

    Davinia Negrín-Báez, Ana Navarro, Juan M. Afonso & María J. Zamorano

  2. Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera A Coruña Km. 7,5, 28040, Madrid, Spain

    Silvia T. Rodríguez-Ramilo

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  1. Davinia Negrín-Báez
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  2. Ana Navarro
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  3. Silvia T. Rodríguez-Ramilo
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  4. Juan M. Afonso
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  5. María J. Zamorano
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Correspondence to María J. Zamorano.

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Negrín-Báez, D., Navarro, A., Rodríguez-Ramilo, S.T. et al. Identification of Quantitative Trait Loci Associated with the Skeletal Deformity LSK complex in Gilthead Seabream (Sparus aurata L.). Mar Biotechnol 18, 98–106 (2016). https://doi.org/10.1007/s10126-015-9671-7

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