Abstract
Small abalone, Haliotis diversicolor, is naturally distributed along the coastal waters of East Asia from Japan to the Philippines. It is an economically important maricultured species in southern China and Taiwan. Genetic linkage maps for small abalone were constructed using a total of 308 simple sequence repeat markers including 297 novel markers. Segregation data on 96 progeny were genotyped using a pseudo-testcross strategy. Sixteen linkage groups were identified in both female and male maps, consistent with the haploid chromosome number. The female linkage map covered 758.3 cM, with an average interval of 5.2 cM. The male linkage map spanned a total genetic distance of 676.2 cM, with an average interval of 4.5 cM. An integrated map was constructed by incorporating the homologous parental linkage groups, resulting in 16 linkage groups with a total of 762.1 cM. Genome coverage of the integrated linkage map was approximately 80.7%. The genetic linkage maps of small abalone may facilitate marker-assisted selection and quantitative trait loci mapping.
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Acknowledgments
The project was supported by the National High Technology Research and Development Program of China (2006AA10A407), the Innovative Platform for Science and Technology, Fujian Province (2008N2004), and the earmarked fund for Modern Agro-Industry Technology Research System (nycytx-47).
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Supplemental Table 1
Characterization of 297 novel microsatellite markers genotyped in the mapping family (DOC 724 kb)
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Zhan, X., Fan, F., You, W. et al. Construction of an Integrated Map of Haliotis diversicolor Using Microsatellite Markers. Mar Biotechnol 14, 79–86 (2012). https://doi.org/10.1007/s10126-011-9390-7
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DOI: https://doi.org/10.1007/s10126-011-9390-7