Skip to main content

Advertisement

SpringerLink
Log in

Construction of a Genetic Linkage Map and Mapping of a Female-Specific DNA Marker in Half-Smooth Tongue Sole (Cynoglossus semilaevis)

  • Original Article
  • Published:
Marine Biotechnology Aims and scope Submit manuscript

Abstract

The half-smooth tongue sole (Cynoglossus semilaevis, hereafter, “tongue sole”) is a marine flatfish with great commercial importance for fisheries and aquaculture in China. It has also been a promising model for the study of sex determination mechanisms in fish. Here, we report the construction of a genetic linkage map for the tongue sole, based on 137 markers including 103 AFLP markers, 33 microsatellite markers, and one female-specific DNA marker. Twenty-six linkage groups (LGs) were found. The total map length was 934.6 cM (Kosambi), with an average spacing of 8.4 cM, covering 64.4% of the estimated genome size. Furthermore, a female-specific SCAR marker, CseF-382, was mapped on LG5. This study represents the first genetic linkage map in the tongue sole. This map has great potential in the identification of quantitative traits loci and sex-related genes and marker-assisted selection in the tongue sole. Meanwhile, the new set of polymorphic microsatellite markers developed in this study is not only useful for genetic mapping but also of critical importance for studies on genetic diversity and broodstock management in tongue sole.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Benson G (1999) Tandem Repeats Finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580

    Article  CAS  PubMed  Google Scholar 

  • Bouza C, Hermida M, Pardo BG, Fernández C, Fortes GG, Castro J, Sánchez L, Presa P, Pérez M, Sanjuán A, de Carlos A, Alvarez-Dios JA, Ezcurra S, Cal RM, Piferrer F, Martínez P (2007) A microsatellite genetic map of the turbot (Scophthalmus maximus). Genetics 177:2457–2467

    Article  CAS  PubMed  Google Scholar 

  • Chakravarti A, Lasher LK, Reefer JE (1991) A maximum likelihood method for estimating genome length using genetic linkage data. Genetics 128:175–182

    CAS  PubMed  Google Scholar 

  • Chen SL, Li J, Deng SP, Tian YS, Wang QY, Zhuang ZM, Shan ZX, Xu JY (2007) Isolation of female-specific AFLP markers and molecular identification of genetic sex in half-smooth tongue sole (Cynoglossus semilaevis). Mar Biotechnol 9:273–280

    Article  CAS  PubMed  Google Scholar 

  • Chen SL, Deng SP, Ma HY, Tian YS, Xu JY, Yang JF, Wang QY, Ji XS, Shao CW, Wang XL, Wu PF, Deng H, Zhai JM (2008a) Molecular marker-assisted sex control in half-smooth tongue sole (Cynoglossus semilaevis). Aquaculture 283:7–12

    Article  CAS  Google Scholar 

  • Chen SL, Tian YS, Yang JF, Shao CW, Ji XS, Zhai JM, Liao XL, Zhuang ZM, Su PZ, Xu JY, Sha ZX, Wu PF, Wang N (2008b) Artificial gynogenesis and sex determination in half-smooth tongue sole (Cynoglossus semilaevis). Mar Biotechnol doi: 10.1007/s10126-008-9139-0

  • Coimbra MRM, Kobayashi K, Koretsugu S, Hasegawa O, Ohara E, Ozaki A, Sakamoto T, Naruse K, Okamoto N (2003) A genetic linkage map of the Japanese flounder, Paralichtus olivaceus. Aquaculture 220:203–218

    Article  CAS  Google Scholar 

  • Danzmann RG, Gharbi K (2001) Gene mapping in fishes: a means to an end. Genetica 111:3–23

    Article  CAS  PubMed  Google Scholar 

  • Danzmann RG, Gharbi K (2007) Linkage mapping in aquaculture species. In: Liu ZJ (ed) Aquaculture genome technologies. Blackwell, Oxford

    Google Scholar 

  • Deng SP (2007) Molecular cloning, characterization and expression analysis of sex-related gene of P450aromatase, FTZ-F1 and DMRT1 in half-smooth tongue-sole, Cynoglossus semilaevis Günther. Doctoral dissertation, Ocean University of China, Qingdao, China

  • Faris JD, Laddomada B, Gill BS (1998) Molecular mapping of segregation distortion loci in Aegilops taushii. Genetics 149:319–327

    CAS  PubMed  Google Scholar 

  • Fishman L, Kelly AJ, Morgan E, Willis JH (2001) A genetic map in the Mimulus guttatus species complex reveals transmission ratio distortion due to heterospecific interactions. Genetics 159:1701–1716

    CAS  PubMed  Google Scholar 

  • Fuji K, Kobayashi K, Hasegawa O, Coimbra MRM, Sakamoto T, Okamoto N (2006) Identification of a single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder (Paralichthys olivaceus). Aquaculture 254:203–210

    Article  CAS  Google Scholar 

  • Haidle L, Janssen JE, Gharbi K, Moghadam HK, Ferguson MM, Danzmann RG (2008) Determination of quantitative trait loci (QTL) for early maturation in rainbow trout (Oncorhynchus mykiss). Mar Biotechnol 10:579–592

    Article  CAS  PubMed  Google Scholar 

  • Johnson NA, Vallejo RL, Silverstein JT, Welch TJ, Wiens GD, Hallerman EM, Palti Y (2008) Suggestive association of major histocompatibility IB genetic markers with resistance to bacterial cold water disease in rainbow trout (Oncorhynchus mykiss). Mar Biotechnol 10:429–437

    Article  CAS  PubMed  Google Scholar 

  • Kang JH, Kim WJ, Lee WJ (2008) Genetic linkage map of olive flounder, Paralichthys olivaceus. Int J Biol Sci 4:143–149

    CAS  PubMed  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Li L, Guo X (2004) AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg. Mar Biotechnol 6:26–36

    Article  CAS  PubMed  Google Scholar 

  • Liao M, Zhang L, Yang G, Zhu M, Wang D, Wei Q, Zou G, Chen D (2007a) Development of silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis) genetic maps using microsatellite and AFLP markers and a pseudo-testcross strategy. Anim Genet 38:364–370

    Article  CAS  PubMed  Google Scholar 

  • Liao X (2006) Development of molecular markers and population genetic analyses in several important fishes distributed in the Yangtze River system. Doctoral dissertation, Institute of Hydrobiology. Chinese Academy of Sciences, Wuhan, China, pp 47

  • Liao X, Shao CW, Tian YS, Chen SL (2007b) Polymorphic dinucleotide microsatellites in tongue sole (Cynoglossus semilaevis). Mol Ecol Notes 7:1147–1149

    Article  CAS  Google Scholar 

  • Liao X, Wang D, Yu X, Li W, Cheng L, Wang J, Tong J (2007c) Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely related species in Gobioninae. Conserv Genet 8:1003–1007

    Article  CAS  Google Scholar 

  • Liu Y, Bao B, Liu L, Wang L, Lin H (2008) Isolation and characterization of polymorphic microsatellite loci from RAPD product in half-smooth tongue sole (Cynoglossus semilaevis) and a test of cross-species amplification. Mol Ecol Resources 8:202–204

    Article  CAS  Google Scholar 

  • Liu Y, Sun X, Gao H, Liu L (2007) Microsatellite markers from an expressed sequence tag library of half-smooth tongue sole (Cynoglossus semilaevis) and their application in other related fish species. Mol Ecol Notes 7:1242–1244

    Article  CAS  Google Scholar 

  • Liu Z, Karsi A, Li P, Cao D, Dunham R (2003) An AFLP-based genetic linkage map of channel catfish (Ictalurus punctatus) constructed by using an interspecific hybrid resource family. Genetics 165:687–694

    CAS  PubMed  Google Scholar 

  • Liu ZJ, Cordes JF (2004) DNA marker technologies and their applications in aquaculture genetics. Aquaculture 238:1–37

    Article  CAS  Google Scholar 

  • Lyttle TW (1991) Segregation distorters. Annu Rev Genet 25:511–557

    Article  CAS  PubMed  Google Scholar 

  • Ning Y, Liu X, Wang Z, Guo W, Li Y, Xie F (2007) A genetic map of large yellow croaker Pseudosciaena crocea. Aquaculture 264:16–26

    Article  CAS  Google Scholar 

  • Reid DP, Smith CA, Rommens M, Blanchard B, Martin-Robichaud D, Reith M (2007) A genetic linkage map of Atlantic halibut (Hippoglossus hippoglossus L.). Genetics 177:1193–1205

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Manitaris T (1989) Molecular cloning: a laboratory manual, ed. 2. Cold Spring Harbor Laboratory Press, Plainview

    Google Scholar 

  • Seitz AC (2008) Robin N. Gibson (Ed.), Flatfishes: biology and exploitation. Rev Fish Biol Fisheries 18:249–250

    Article  Google Scholar 

  • Staelens J, Rombaut D, Vercauteren I, Argue B, Benzie J, Vuylsteke M (2008) High-density linkage maps and sex-linked markers for the black tiger shrimp (Penaeus monodon). Genetics 179:917–925

    Article  CAS  PubMed  Google Scholar 

  • Tan YD, Wan C, Zhu Y, Lu C, Xiang Z, Deng HW (2001) An amplified fragment length polymorphism map of the silkworm. Genetics 157:1277–1284

    CAS  PubMed  Google Scholar 

  • Tong J, Chu KH (2002) Genome mapping in aquatic animals: progress and future perspectives. Russ J Genet 38:612–621

    Article  CAS  Google Scholar 

  • van Ooijen JW, Voorrips RE (2001) JoinMap® 3.0, software for the calculation of genetic linkage maps. Plant Research International, Wageningen

    Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  CAS  PubMed  Google Scholar 

  • Wang D, Liao X, Cheng L, Yu X, Tong J (2007) Development of novel EST-SSR markers in common carp by data mining from public EST sequences. Aquaculture 271:558–574

    Article  CAS  Google Scholar 

  • Wang X, Zhang Q, Sun X, Chen Y, Zhai T, Zhuang W, Qi J, Wang Z (2008a) Fosmid library construction and initial analysis of end sequences in female half-smooth tongue sole (Cynoglossus semilaevis). Mar Biotechnol. doi:10.1007/s10126-008-9137-2

  • Wang X, Zhang Q, Sun X, Yin G, Qi J, Wang Z, Wang X (2008b) Isolation and characterization of 64 novel microsatellite markers from a fosmid library of female half-smooth tongue sole (Cynoglossus semilaevis). Mol Ecol Res 8:1303–1306

    Article  CAS  Google Scholar 

  • Yu Z, Guo X (2006) Identification and mapping of disease resistance QTLs in the eastern oyster, Crassostrea virginica Gmelin. Aquaculture 254:160–170

    Article  CAS  Google Scholar 

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  CAS  PubMed  Google Scholar 

  • Zhou LQ, Yang AG, Liu XZ, Du W, Zhuang ZM (2005) The karyotype analysis of Cynoglossus semilaevis in China. J Fish China 29:417–419 (in Chinese with English abstract)

    Google Scholar 

  • Zhuang ZM, Wu D, Zhang SC, Pang QX, Wang CL, Wan RJ (2006) G-banding patterns of the chromosomes of tonguefish Cynoglossus semilaevis Günther, 1873. J Appl Ichthyol 22:437–440

    Article  Google Scholar 

Download references

Acknowledgements

We are grateful to Dr. Meijie Liao for data analysis and Miss Yini Jiang for partial microsatellite genotyping. This work was supported by 863 Project (2006AA10A403), Shandong Genetic Improvement Key Project for Agricultural Organism, Taishan Scholar Project of Shandong Province, and Scientific Foundation of YSFRI, CAFS (2006-qn-13).

Author information

Authors and Affiliations

  1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Xiaolin Liao, Hong-Yu Ma, Gen-Bo Xu, Chang-Wei Shao, Yong-Sheng Tian, Xiang-Shan Ji, Jing-Feng Yang & Song-Lin Chen

Authors
  1. Xiaolin Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hong-Yu Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gen-Bo Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chang-Wei Shao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yong-Sheng Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiang-Shan Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jing-Feng Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Song-Lin Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Song-Lin Chen.

Additional information

Xiaolin Liao and Hongyu Ma contributed equally to the article.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liao, X., Ma, HY., Xu, GB. et al. Construction of a Genetic Linkage Map and Mapping of a Female-Specific DNA Marker in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Mar Biotechnol 11, 699–709 (2009). https://doi.org/10.1007/s10126-009-9184-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10126-009-9184-3

Keywords

Search

Navigation