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QTL Mapping for Frond Length and Width in Laminaria japonica Aresch (Laminarales, Phaeophyta) Using AFLP and SSR Markers

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Abstract

In Laminaria japonica Aresch breeding practice, two quantitative traits, frond length (FL) and frond width (FW), are the most important phenotypic selection index. In order to increase the breeding efficiency by integrating phenotypic selection and marker-assisted selection, the first set of QTL controlling the two traits were determined in F2 family using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. Two prominent L. japonicas inbred lines, one with “broad and thin blade” characteristics and another with “long and narrow blade” characteristics, were applied in the hybridization to yield the F2 mapping population with 92 individuals. A total of 287 AFLP markers and 11 SSR markers were used to construct a L. japonica genetic map. The yielded map was consisted of 28 linkage groups (LG) named LG1 to LG28, spanning 1,811.1 cM with an average interval of 6.7 cM and covering the 82.8% of the estimated genome 2,186.7 cM. While three genome-wide significant QTL were detected on LG1 (two QTL) and LG4 for “FL,” explaining in total 42.36% of the phenotypic variance, two QTL were identified on LG3 and LG5 for the trait “FW,” accounting for the total of 36.39% of the phenotypic variance. The gene action of these QTL was additive and partially dominant. The yielded linkage map and the detected QTL can provide a tool for further genetic analysis of two traits and be potential for maker-assisted selection in L. japonica breeding.

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Acknowledgement

The authors want to thank Donald Sturge for the English revision of the manuscript and also acknowledge the significant contributions of the anonymous reviewers. This research was supported by Key Lab of Marine Bioactive Substances, State Oceanography Administration, and funded by Natural Science Foundation of China No. 30500383 to X. L. Wang, and Knowledge innovation program (KSCXZ-YW-N-47-02) of Chinese Academy of Sciences and Shandong Agricultural Seed Stock Breeding Project granted to D. L. Duan.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Fuli Liu, Zhanru Shao, Haining Zhang, Jidong Liu, Xiuliang Wang & Delin Duan

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100059, China

    Fuli Liu, Zhanru Shao & Haining Zhang

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  1. Fuli Liu
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  2. Zhanru Shao
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  3. Haining Zhang
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  4. Jidong Liu
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  5. Xiuliang Wang
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  6. Delin Duan
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Correspondence to Xiuliang Wang or Delin Duan.

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Liu, F., Shao, Z., Zhang, H. et al. QTL Mapping for Frond Length and Width in Laminaria japonica Aresch (Laminarales, Phaeophyta) Using AFLP and SSR Markers. Mar Biotechnol 12, 386–394 (2010). https://doi.org/10.1007/s10126-009-9229-7

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