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Implications of the Up-regulation of Genes Encoding Protein Degradation Enzymes and Heat Shock Protein 90 for Intertidal Green Macroalga Ulva fasciata Against Hypersalinity-Induced Protein Oxidation

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Abstract

The molecular acclimation of intertidal green macroalga Ulva fasciata Delile to high salinity stress were examined by the construction of a forward cDNA library via the suppressive subtractive hybridization between 30‰ and 90‰ (24 h) and by the time course dynamics of several abundantly expressed genes. Among the genes with known sequences, the expressed sequence tags are abundant in the function of protein synthesis (ribosomal protein) and destination. The cDNAs of ATP-dependent Clp protease (UfClpC), 20S proteasome β-subunit type 1 domain (UfPbf1), ubiquitin-conjugating enzyme E2 I (UfUbc9), and heat shock protein 90A (UfHsp90A) were cloned. UfClpC transcript increased 3 h after 90‰ treatment, followed by a decrease, while UfPbf1 and UfUbc9 transcripts increased after 12 h and decreased at 48 h. The transcripts of UfHsp90A increased 1 h after 90‰ treatment, followed by a drop and to the control level at 48 h. Protease activity increased 3 h after 90‰ treatment and decreased to the control level at 48 h. H2O2 contents increased 1 h after 90‰ treatment and then remained unchanged, but protein carbonyl group contents increased after 48 h. The treatments of reactive oxygen species scavengers partially alleviated 90‰ damage (partial growth rescue) and suppressed the increases in H2O2 content, protein carbonyl group content, protease activity, and UfClpC, UfPbf1, UfUbc9, and UfHsp90A transcripts by 90‰. The induction of specific chaperones and proteases at the molecular level for protein quality control can be considered as one of the molecular mechanisms of hypersalinity acclimation in U. fasciata.

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Acknowledgments

The grants from the National Science Council, Executive Yuan, Taiwan (NSC 96-2311-B-110- 002-MY3, NSC 97-2311-B-110-002, and NSC 98-2311-B-110-002) and the Kuroshio Research Group of Asia-Pacific Ocean Research Center of National Sun Yat-sen University, funded by the Ministry of Education, Executive Yuan, Taiwan, under the Grant-Aim for the Top University Plan, to Tse-Min Lee are appreciated. A post-doctoral grant from the National Science Council, Executive Yuan, Taiwan, to Dr. Kuan-Lin Ho is also appreciated.

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Authors and Affiliations

  1. Institute of Marine Biology, National Sun Yat-sen University, Kaohsiung, 804, Taiwan, Republic of China

    Ming-Shiuan Sung, Yuan-Ting Hsu, Kuan-Lin Ho & Tse-Min Lee

  2. The Kuroshio Research Group of the Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung, 804, Taiwan, Republic of China

    Ming-Shiuan Sung, Yuan-Ting Hsu, Kuan-Lin Ho & Tse-Min Lee

  3. Doctor Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung, 804, Taiwan, Republic of China

    Ming-Shiuan Sung, Yuan-Ting Hsu, Kuan-Lin Ho & Tse-Min Lee

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  1. Ming-Shiuan Sung
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Supplementary Table S1

Quantitative real-time PCR primers. (DOC 31 kb)

Supplementary Table S2

GSP and NSP primers (DOC 39 kb)

Supplementary Table S3

SSH clones. (DOC 98 kb)

Supplementary Fig. S1

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Supplementary Fig. S2

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Supplementary Fig. S3

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Sung, MS., Hsu, YT., Ho, KL. et al. Implications of the Up-regulation of Genes Encoding Protein Degradation Enzymes and Heat Shock Protein 90 for Intertidal Green Macroalga Ulva fasciata Against Hypersalinity-Induced Protein Oxidation. Mar Biotechnol 13, 684–694 (2011). https://doi.org/10.1007/s10126-010-9330-y

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