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Use of Human cDNA Microarrays for Identification of Differentially Expressed Genes in Atlantic Salmon Liver During Aeromonas salmonicida Infection

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Abstract

Commercially available human complementary DNA microarrays were used to compare differential expression in the livers of Atlantic salmon (Salmo salar) infected with Aeromonas salmonicida and of healthy fish. Complementary DNA probes were prepared from total RNA isolated from livers of control salmon and infected salmon by reverse transcription in the presence of 33P-dCTP and independently hybridized to human GENEFILTERS GF211 microarrays. Of the 4131 known genes on the microarray, 241 spots gave clearly detectable signals using labeled RNA from the control salmon liver. Of these, 4 spots were consistently found to have a greater than 2-fold increase in infected salmon compared with controls when using the same pair of filters to generate hybridization data from triplicates. These up-regulated genes were ADP/ATP translocase (AAT2), Na+/K+ ATPase, acyloxyacyl hydrolase (AOAH), and platelet-derived growth factor (PDFG-A). A BlastN search revealed an AAT2 homolog from Atlantic salmon, and a reverse transcriptase polymerase chain reaction assay using primers based on this sequence confirmed its up-regulation (approx. 1.8-fold) during early infection. This work demonstrates the feasibility of using human microarrays to facilitate the discovery of differentially expressed genes in Atlantic salmon, for which no homologous microarrays are available.

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Acknowledgements

We thank Dr. Andrew Dacanay, Krista Melville, and Sandra Sperker for performing the cohabitation challenge. This work was funded in part by grant HL64601 to Dr. Ian Bird and in part by the National Research Council Genomics and Health Initiative. We thank Dr. John van de Meer and Dr. Stewart Johnson for reviewing the manuscript. This is NRC publication number 42382.

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Authors and Affiliations

  1. National Research Council, Institute for Marine Biosciences, 1411 Oxford Street, Halifax, Nova Scotia B3H 3Z1, Canada

    Stephen C. M. Tsoi, Vanya Ewart, Laura L. Brown & Susan Douglas

  2. Perinatal Research Laboratories, Department of Obstetrics and Gynecology, University of Wisconsin–Madison, Madison, WI 53715, USA

    Jacqueline M. Cale & Ian M. Bird

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  1. Stephen C. M. Tsoi
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  2. Jacqueline M. Cale
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Correspondence to Stephen C. M. Tsoi.

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Tsoi, S.C., Cale, J.M., Bird, I.M. et al. Use of Human cDNA Microarrays for Identification of Differentially Expressed Genes in Atlantic Salmon Liver During Aeromonas salmonicida Infection . Mar. Biotechnol. 5, 545–554 (2003). https://doi.org/10.1007/s10126-002-0112-z

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