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UV-Absorbing Substance in the Red Alga Porphyra yezoensis (Bangiales, Rhodophyta) Block Thymine Photodimer Production

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Abstract

The effect of the water-soluble UV-absorbing substance (UVAS) extracted from the marine red alga Porphyra yezoensis Ueda on UV-dependent thymine photodimer production was investigated. The T<>T pyrimidine-pyrimidone 6-4 dimer and the cyclobutane cis-syn T<>T 5-6 dimer produced by UV irradiation with a xenon lamp were analyzed by reverse-phase high-performance liquid chromatography. Although the dimer production was reduced when the irradiation was filtered through a UVAS solution, it decreased more when thymine was mixed with UVAS. Furthermore, UVAS inhibited the degradation of UV-irradiated thymine. The inhibitory effect of UVAS was significantly greater than that of exogenously added adenine or guanine, which forms complementary base pairs with thymine. These data suggest that in addition to its filtering effect against UV radiation, UVAS also protects thymine by a direct molecule-to-molecule energy transfer process. The protective function of UVAS against UV irradiation is advantageous for this alga under strong UV irradiation.

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Acknowledgements

We are grateful to Professor K. Yabe of Hokkaido Tokai University for the authentic MAA compounds. We are indebted to Professor Y. Yokohama of Tsukuba University for kind help with HPLC analysis, and to Professor M. Mimuro of Yamaguchi University for helpful suggestions on this study. We also thank to Ms. M. Sugiyaina for her technical assistance.

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Authors and Affiliations

  1. Department of Ecosocial System Engineering, Faculty of Engineering, Yamanashi University, Kofu, Yamanashi 400-8511, Japan

    Taku Misonou

  2. Department of Biology, Faculty of Education, Yamanashi University, Kofu, Yamanashi 400-8510, Japan

    Junko Saitoh & Saori Oshiba

  3. Department of Food Science, Faculty of Education and Human Sciences, Yamanashi University, Kofu, Yamanashi 400-8510, Japan

    Yukiko Tokitomo

  4. Department of Marine Biological Science, Faculty of Bioresources, Mie University, Tsu, Mie 514-8507, Japan

    Miyuki Maegawa

  5. Department of Electrical Engineering, Faculty of Engineering, Yamanashi University, Kofu, Yamanashi 400-8511, Japan

    Yukio Inoue, Hirokazu Hori & Takeki Sakurai

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  1. Taku Misonou
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  2. Junko Saitoh
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  4. Yukiko Tokitomo
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  7. Hirokazu Hori
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  8. Takeki Sakurai
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Correspondence to Taku Misonou.

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Misonou, T., Saitoh, J., Oshiba, S. et al. UV-Absorbing Substance in the Red Alga Porphyra yezoensis (Bangiales, Rhodophyta) Block Thymine Photodimer Production . Mar. Biotechnol. 5, 194–200 (2003). https://doi.org/10.1007/s10126-002-0065-2

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  • DOI: https://doi.org/10.1007/s10126-002-0065-2

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