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Effect of systemic photobiomodulation in the course of acute lung injury in rats

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Abstract

Acute lung injury (ALI) is a severe, multifactorial lung pathology characterized by diffuse alveolar injury, inflammatory cell infiltration, alveolar epithelial barrier rupture, alveolar edema, and impaired pulmonary gas exchange, with a high rate of mortality; and sepsis is its most common cause. The mechanisms underlying ALI due to systemic inflammation were investigated experimentally by systemic lipopolysaccharide (LPS) administration. Photobiomodulation (PBM) has been showing good results for several inflammatory diseases, but there are not enough studies to support the real benefits of its use, especially systemically. Considering that ALI is a pathology with high morbidity and mortality, we studied the effect of systemic PBM with red light-emitting diode (LED) (wavelength 660 nm; potency 100 mW; energy density 5 J/cm; total energy 15 J; time 150 s) in the management of inflammatory parameters of this disease. For this, 54 male Wistar rats were submitted to ALI by LPS injection (IP) and treated or not with PBM systemically in the tail 2 and 6 h after LPS injection. Data were analyzed by one-way ANOVA followed by Student’s Newman-Keuls. Our results point to the beneficial effects of systemic PBM on the LPS-induced ALI, as it reduced the number of neutrophils recruited into the bronchoalveolar lavage, myeloperoxidase activity, and also reduced interleukins (IL) 1β, IL-6, and IL-17 in the lung. Even considering the promising results, we highlight the importance of further studies to understand the mechanisms involved, and especially the dosimetry, so that in near future, we can apply this knowledge in clinical practice.

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Funding

This study was sponsored by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP: 2017/0644-9) and CNPq 305099/2017-5.

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Authors and Affiliations

  1. Post Graduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE), Rua Vergueiro, 239/245, São Paulo, SP, CEP 01504-000, Brazil

    João Gabriel Fernandes da Silva, Sabrina Soares dos Santos, Patricia de Almeida, Rodrigo Labat Marcos & Adriana Lino-dos-Santos-Franco

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  1. João Gabriel Fernandes da Silva
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  2. Sabrina Soares dos Santos
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  3. Patricia de Almeida
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  4. Rodrigo Labat Marcos
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  5. Adriana Lino-dos-Santos-Franco
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Corresponding author

Correspondence to Adriana Lino-dos-Santos-Franco.

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The authors declare that they have no conflicts of interest.

Ethical approval

The animals were obtained from the University Nove de Julho and the experiments were approved by the Animal Care Committee University Nove de Julho (CoEP-UNINOVE, AN0006/2017). The methods used in the present study were carried out in accordance with the relevant guidelines.

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da Silva, J.G.F., dos Santos, S.S., de Almeida, P. et al. Effect of systemic photobiomodulation in the course of acute lung injury in rats. Lasers Med Sci 36, 965–973 (2021). https://doi.org/10.1007/s10103-020-03119-7

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  • DOI: https://doi.org/10.1007/s10103-020-03119-7

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