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Isolation and identification of antiproliferative substances from ginseng fermented using Ganoderma lucidum mycelia

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Abstract

Two active substances from ginseng fermented using Ganoderma lucidum mycelia were investigated for antiproliferative effects against the human lung cancer cell line A549. The chloroform fraction of fermented ginseng extracts showed a strong antiproliferative effect. This fraction was isolated and purified using silica gel and C18 resin column chromatography and semi-preparative reverse phase HPLC. The structures of isolated compounds were determined using spectroscopic methods (ESI-MS, 1H and 13C NMR). Isolated compounds were identified as ginsenoside compound K and 3-oxo-compound K. Both inhibited A549 cell growth in a dose-dependent manner. Cell viability values for ginsenoside compound K were 74.88, 59.30, 5.76, 5.79, and 6.27% at 6.25, 12.50, 25.00, 50.00, and 100.00 μg/mL, respectively, and ginsenoside 3-oxo-compound K showed values 89.40, 59.62, 6.05, and 4.64% at 3.70, 7.50, 15.00, and 30.00 μg/mL, respectively. Compound K and 3-oxo-compound K from fermented ginseng can be used as natural anti-cancer agents.

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References

  1. Cheng Y, Lee S, Lin S, Chang W, Chen Y, Tsai N, Liu Y, Tzao C, Yu S, Ham H. Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo. Cancer Lett. 222: 183–193 (2005)

    Article  CAS  Google Scholar 

  2. Manosroi J, Sainakham M, Manosroi W, Manosroi A. Antiproliferative and apoptosis induction activities of extracts from thai medicinal plant recipes selected from MANOSROI II database. J. Ethnopharmacol. 141: 451–459 (2012)

    Article  Google Scholar 

  3. Yu HE, Cho SM, Seo GS, Lee BS, Lee DH, Lee JS. Screening of bioactive compounds from mushroom Pholiota sp. Korean J. Mycol. 34: 15–21 (2006)

    Article  Google Scholar 

  4. Oh SI, Lee MS. Antioxidative and antimutagenic effects of Ganoderma lucidum krast extracts. Korean J. Food Nutr. 18: 54–62 (2005)

    Google Scholar 

  5. Jin H, Jin F, Jin JX, Xu J, Tao TT, Liu J, Huang HJ. Protective effects of Ganoderma lucidum spore on cadmium hepatotoxicity in mice. Food Chem. Toxicol. 52: 171–175 (2012)

    Article  Google Scholar 

  6. Ha CL. The inhibitory effect of the Chinese herb Ganoderma lucidum mycelium on gut immunoglobulin A responses to cholera toxin in mice. Nutr. Res. 23: 691–701 (2003)

    Article  CAS  Google Scholar 

  7. Hu SY. The genus Panax (ginseng) in Chinese medicine. Econ. Bot. 30: 11–28 (1976)

    Article  Google Scholar 

  8. Kim DC, Hwang WI, In MJ, Lee SD. Effects of lipid soluble ginseng extract on immune response. J. Ginseng Res. 32: 19–25 (2008)

    Article  Google Scholar 

  9. Lee MH, Choi SW, Kim EJ. Differential anti-carcinogenic effect of mountain cultivated ginseng and ginseng on mouse skin carcinogenesis. J. Korean Soc. Food Sci. Nutr. 41: 462–470 (2012)

    Article  CAS  Google Scholar 

  10. Kim HS, Lee MK, Back DY, Hwang SL, Choi KJ, Kim SC. Effects of ginsenosides on the development of morphine-induced tolerance and physical dependence in mice. Korean J. Pharmacogn. 20: 123–127 (1989)

    CAS  Google Scholar 

  11. Tchilian EZ, Zhelezarov IE, Hadjiivanova CI. Effect of ginsenoside Rg1 on insulin binding in mice liver and brain membranes. Phytother. Res. 5: 46–48 (1991)

    Article  Google Scholar 

  12. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee JS, Jeong HS. Change of Korean ginseng components with high temperature and pressure treatment. Korean J. Food Sci. Technol. 38: 521–525 (2006)

    Google Scholar 

  13. Joung EM, Kim HY, Hwang IG, Jeong JH, Yu KW, Lee JS, Jeong HS. Changes of antioxidant activities on cultured ginseng with mushroom mycelia during cultivation. J. Korean Soc. Food Sci. Nutr. 39: 1346–1352 (2010)

    Article  Google Scholar 

  14. Joung EM, Hwang IG, Lee HY, Jeong JH, Yu KW, Jeong HS. Changes of saponin and β-glucan content on the cultured ginseng with mushroom mycelia. J. Korean Soc. Food Sci. Nutr. 38: 1084–1089 (2009)

    Article  CAS  Google Scholar 

  15. Kim HY, Joung EM, Hwang IG, Jeong JH, Yu KW, Lee JS, Jeong HS. Effect of fermented ginseng extract by mushroom mycelia on antiproliferation of cancer cells. J. Korean Soc. Food Sci. Nutr. 39: 36–41 (2010)

    Article  CAS  Google Scholar 

  16. Kim HO, Park MJ, Han JS. Effects of fermented red ginseng supplementation on blood glucose and insulin resistance in type 2 diabetic patients. J. Korean Soc. Food Sci. Nutr. 40: 696–703 (2011)

    Article  CAS  Google Scholar 

  17. Bae SH, Lee HS, Kim MR, Kim SY, Kim JM, Suh HJ. Changes of ginsenoside content by mushroom mycelial fermentation in red ginseng extract. J. Ginseng Res. 35: 235–242 (2011)

    Article  CAS  Google Scholar 

  18. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red, and crystal violet. Biol. Pharm. Bull. 19: 1518–1520 (1996)

    Article  CAS  Google Scholar 

  19. Park EK, Shin YW, Lee HU, Kim SS, Lee YC, Lee BY, Kim DH. Inhibitory effect of ginsenoside Rb1 and compound K on NO and prostaglandin E2 biosyntheses of RAW 264.7 cells induced by lipopolysaccharide. Biol. Pharm. Bull. 28: 652–656 (2005)

    Article  CAS  Google Scholar 

  20. Han GC, Ko SK, Sung JH, Chung SH. Compound K enhances insulin secretion with beneficial metabolic effects in db/db mice. J. Agr. Food Chem. 55: 10641–10648 (2007)

    Article  CAS  Google Scholar 

  21. Surh YJ, Na HK, Lee JY, Keum YS. Molecular mechanisms underlying anti-tumor promoting activities of heat-processed Panax ginseng C.A. Meyer. J. Korean Med. Sci. 16: S38–S41 (2001)

    Article  CAS  Google Scholar 

  22. Choi KS, Choi CH. Proapoptotic ginsenosides compound K and Rh2 enhance Fas-induced cell death of human astrocytoma cells through distinct apoptotic signaling pathways. Cancer Res. Treat. 41: 36–44 (2009)

    Article  Google Scholar 

  23. Choi YS, Han GC, Han EJ, Park KJ, Sung JH, Chung SH. Effects of compound K on insulin secretion and carbohydrate metabolism. J. Ginseng Res. 31: 79–85 (2007)

    Article  CAS  Google Scholar 

  24. Yoon SH, Han EJ, Sung JH, Chung SH. Anti-diabetic effects of compound K versus metformin versus compound K-metformin combination therapy in diabetic db/db mice. Biol. Pharm. Bull. 30: 2196–2200 (2007)

    Article  CAS  Google Scholar 

  25. Li W, Zhang M, Gu J, Meng ZJ, Zhao LC, Zheng YN, Chen L, Yang GL. Hypoglycemic effect of protopanaxadiol-type ginsenosides and compound K on type 2 diabetes mice induced by high-fat diet combining with streptozotocin via suppression of hepatic gluconeogenesis. Fitoterapia 83: 192–198 (2012)

    Article  CAS  Google Scholar 

  26. Shin YW, Kim DH. Antipruritic effect of ginsenoside Rb1 and compound K in scratching behavior mouse models. J. Pharmacol. Sci. 99: 83–88 (2005)

    Article  CAS  Google Scholar 

  27. Lee HU, Bae EA, Han MJ, Kim NJ, Kim DH. Hepatoprotective effect of ginsenoside Rb1 and compound K on tert-butyl hydroperoxide-induced liver injury. Liver Int. 25: 1069–1073 (2005)

    Article  CAS  Google Scholar 

  28. Park DM, Yoon MC. Compound K, a novel ginsenoside metabolite, inhibits adipocyte differentiation in 3T3-L1 cells: Involvement of angiogenesis and MMPs. Biochem. Bioph. Res. Co. 422: 263–267 (2012)

    Article  CAS  Google Scholar 

  29. Chen GT, Yang M, Song Y, Lu ZQ, Zhang JQ, Huang HL, Wu LI, Guo DA. Microbial transformation of ginsenoside Rb1 by Acremonium strictum. Appl. Microbiol. Biot. 77: 1345–1350 (2008)

    Article  CAS  Google Scholar 

  30. Quan LH, Liang ZQ, Kim HB, Kim SH, Kim SY, Noh YD, Yang DC. Conversion of ginsenoside Rd to compound K by crude enzymes extracted from Lactobacillus brevis LH8. J. Ginseng Res. 32: 226–231 (2008)

    Article  CAS  Google Scholar 

  31. Noh KH, Son JW, Kim HJ, Oh DK. Ginsenoside compound K production from ginseng root extract by a thermostable betaglycosidase from Sulfolobus solfataricus. Biosci. Biotech. Bioch. 73: 316–321 (2009)

    Article  CAS  Google Scholar 

  32. Bae EA, Kim NY, Han MJ, Choo MK, Kim DH. Transformation of ginsenosides to compound K (IH-901) by lactic acid bacteria of human intestine. J. Microbiol. Biotechn. 13: 9–14 (2003)

    CAS  Google Scholar 

  33. Piao JY, Kim YJ, Quan LH, Yang DU, Min JW, Son SH, Kim SM, Yang DC. Bioconversion of ginsenoside Rb1 to compound K using Leuconostoc lactis DC201. Korean J. Plant Res. 24: 712–718 (2011)

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea

    Gwi Yeong Jang, Eun Mi Joung, Sang Hoon Lee, Junsoo Lee & Heon Sang Jeong

  2. Department of Food and Nutrition, Korea National University of Transportation, Jeungpyeong, Chungbuk, 368-701, Korea

    Jae-Hyun Jeong

  3. College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea

    Bang Yeon Hwang & Jin Tae Hong

Authors
  1. Gwi Yeong Jang
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  2. Eun Mi Joung
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  3. Sang Hoon Lee
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  4. Jae-Hyun Jeong
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  7. Junsoo Lee
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Correspondence to Heon Sang Jeong.

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Jang, G.Y., Joung, E.M., Lee, S.H. et al. Isolation and identification of antiproliferative substances from ginseng fermented using Ganoderma lucidum mycelia. Food Sci Biotechnol 24, 567–574 (2015). https://doi.org/10.1007/s10068-015-0074-3

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  • DOI: https://doi.org/10.1007/s10068-015-0074-3

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