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Lipase digestibility of the oil phase in a water-in-oil-in-water emulsion

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Abstract

Hydrolysis of the oil phase in a water-in-oil-in-water (W/O/W) emulsion was studied using pancreatic lipase. The influence of the W/O volume fraction, lipase concentration, and concentration of emulsifiers used to prepare W/O/W emulsions on lipid digestion and release of encapsulated material were investigated. The extent of lipid digestion differed depending on the W/O volume and lipase concentration, and the amount of dye released was not dependent on the W/O content or lipase concentration. The concentration of emulsifiers did not affect the extent of digestion. The information obtained in this study will be useful for design of W/O/W emulsion formulations with controlled release profiles and lipid digestion.

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References

  1. Matalanis A, Jones OG, McClements DJ. Structured biopolymer-based delivery systems for encapsulation, protection, and release of lipophilic compounds. Food Hydrodolloid. 25: 1865–1880 (2011)

    Article  CAS  Google Scholar 

  2. Matalanis A, McClement DJ. Impact of encapsulation within hydrogel microspheres on lipid digestion: An in vitro study. Food Biophys. 7: 145–154 (2012)

    Article  Google Scholar 

  3. McClements DJ, Decker EA, Park Y. Controlling lipid bioavailability through physicochemical and structural approaches. Crit. Rev. Food Sci. 49: 48–67 (2009)

    Article  Google Scholar 

  4. Qian C, Decker EA, Xiao H, McClements DJ. Impact of lipid nanoparticle physical state on particle aggregation and β-carotene degradation: Potential limitations of solid lipid nanoparticles. Food Res. Int. 52: 342–349 (2013)

    Article  CAS  Google Scholar 

  5. Singh H, Ye AQ, Horne D. Structuring food emulsions in the gastrointestinal tract to modify lipid digestion. Prog. Lipid Res. 48: 92–100 (2009)

    Article  CAS  Google Scholar 

  6. Troncoso E, Aguilera JM, McClements DJ. Fabrication, characterization and lipase digestibility of food-grade nanoemulsion. Food Hydrocolloid. 27: 355–363 (2012)

    Article  CAS  Google Scholar 

  7. Leal-Calderon F, Homer S, Goh A, Lundin L. W/O/W emulsions with high internal droplet volume fraction. Food Hydrocolloid. 27: 30–41 (2012)

    Article  CAS  Google Scholar 

  8. Garti N. Double emulsions-scope, limitations, and new achievements. Colloid. Surface. A. 123–124: 233–246 (1997)

    Article  Google Scholar 

  9. Benichou A, Aserin A, Garti N. Double emulsions stabilized with hybrids of natural polymers for entrapment and slow release of active matters. Adv. Colloid Interfac. 108–109: 29–41 (2004)

    Article  Google Scholar 

  10. Benichou A, Aserin A, Garti N. W/O/W double emulsions stabilized with WPI-polysaccharide complexes. Colloid. Surface. A. 294: 20–32 (2007)

    Article  CAS  Google Scholar 

  11. Weiss J, Scherze I, Muschiolik G. Polysaccharide gel with multiple emulsion. Food hydrocolloid. 19: 605–615 (2005)

    Article  CAS  Google Scholar 

  12. Su J, Flanagan J, Hemar Y, Singh H. Synergistic effects of polyglycerol ester of polyricinoleic acid and sodium caseinate on the stabilisation of water-oil-water emulsions. Food Hydrocolloid. 20: 261–268 (2006)

    Article  CAS  Google Scholar 

  13. Silva-Cunha A, Grossiord JL, Puisieux F, Seiller M. Insulin in w/o/w multiple emulsions: Preparation, characterization, and determination of stability towards protease in vitro. J. Microencapsul. 14: 311–319 (1997)

    Article  Google Scholar 

  14. Silva-Cunha A, Grossiord JL, Puisieux F, Seiller M. Insulin in w/o/w multiple emulsions: Biological activity after oral administration in normal and diabetic rats. J. Microencapsul. 14: 321–333 (1997)

    Article  CAS  Google Scholar 

  15. Silva-Cunha A, Chéron M, Grossiord JL, Puisieux F, Seiller M. W/O/W multiple emulsions of insulin containing a protease inhibitor and an absorption enhancer: Biological activity after oral administration to normal and diabetic rats. Int. J. Pharm. 169: 33–44 (1998)

    Article  CAS  Google Scholar 

  16. Surh J, Vladisavljeviæ GT, Mun SH, McClements DJ. Preparation and characterization of water/oil and water/oil/water emulsions containing biopolymer-gelled water droplets. J. Agr. Food Chem. 55: 175–184 (2007)

    Article  CAS  Google Scholar 

  17. Tokgoz NS, Grossiord JL, Fructus A, Seiller M, Prognon P. Evaluation of two fluorescent probes for the characterization of W/O/W emulsions. Int. J. Pharm. 141: 27–37 (1996)

    Article  CAS  Google Scholar 

  18. Adachi S, Imaoka H, Hasegawa Y, Matsuno R. Preparation of a water-in-oil-in-water (W/O/W) type microcapsules by a single-droplet-drying method and change in encapsulation efficiency of a hydrophilic substance during storage. Biosci. Biotech. Bioch. 67: 1376–1381 (2003)

    Article  CAS  Google Scholar 

  19. Li Y, Hu M, McClements DJ. Factors affecting lipase digestibility of emulsified lipids using an in vitro digestion model: Proposal for a standardised pH-stat method. Food Chem. 126: 498–505 (2011)

    Article  CAS  Google Scholar 

  20. Mun S, Decker EA, McClements DJ. Influence of emulsifier type on in vitro digestibility of lipid droplets by pancreatic lipase. Food Res. Int. 40: 770–781 (2007)

    Article  CAS  Google Scholar 

  21. Nik AM, Wright AJ, Corredig M. Impact of interfacial composition on emulsion digestion and rate of lipid hydrolysis using different in vitro digestion models. Colloid. Surface. B. 83: 321–330 (2011)

    Article  Google Scholar 

  22. Euston SR, Baird WG, Campbell L, Kuhns M. Competitive adsorption of dihydroxy and trihydroxy bile salts with whey protein and casein in oil-in-water emulsions. Biomacromolecules 14: 1850–1858 (2013)

    Article  CAS  Google Scholar 

  23. Bellesi FA, Ruiz-Henestrosa VMP, Pilosof AMR. Behavior of protein interfacial films upon bile salts addition. Food Hydrocolloid. 36: 115–122 (2014)

    Article  CAS  Google Scholar 

  24. McClements DJ. Food emulsions: Principles, practices, and techniques. CRC Press, Boca Raton, FL, USA. pp. 310–324 (2005)

    Google Scholar 

  25. Ahmed K, Li Y, McClements DJ, Xiao H. Nanoemulsion-and emulsion-based delivery systems for curcumin: Encapsulation and release properties. Food Chem. 132: 799–807 (2012)

    Article  CAS  Google Scholar 

  26. Salvia-Trujillo L, Qian C, Martín-Belloso O, McClements DJ. Modulating β-carotene bioaccessibility by controlling oil composition and concentration in edible nanoemulsions. Food Chem. 139: 878–884 (2013)

    Article  CAS  Google Scholar 

  27. Hu M, Li Y, Decker EA, McClements DJ. Role of calcium and calcium-binding agents on the lipase digestibility of emulsified lipids using an in vitro digestion model. Food Hydrocolloid. 24: 719–725 (2010)

    Article  CAS  Google Scholar 

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Correspondence to Yong-Ro Kim.

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Mun, S., Choi, Y. & Kim, YR. Lipase digestibility of the oil phase in a water-in-oil-in-water emulsion. Food Sci Biotechnol 24, 513–520 (2015). https://doi.org/10.1007/s10068-015-0067-2

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  • DOI: https://doi.org/10.1007/s10068-015-0067-2

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