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Fermentation kinetics of production of ubiquinone-10 by Paracoccus dinitrificans NRRL B-3785: Effect of type and concentration of carbon and nitrogen sources

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Abstract

Ubiquinone-10 (CoQ10), a vitamin-like lipophilic component of the membrane-bound electron transport system, has a wide range of therapeutic, neutraceutical, and cosmeceutical applications. The objective of this study was to optimize nutritional requirements for production of CoQ10 by Paracoccus dinitrificans NRRL B-3785 fermentation. Effect of type and concentration of carbon and nitrogen source on fermentation kinetic parameters were analyzed using logistic and Luedeking-Piret equations. In submerged batch fermentation, yield of CoQ10 was 12.22 mg/L when 40 g/L glycerol was used and specific growth rate (0.056/h) as well as growth associated constant (α=0.680 mg/g) were higher as compared to other concentrations. Ammonium nitrate and proteose peptone at 5 (α=0.677 mg/g; β= 0.0072 mg/g·h) and 20 g/L (α=0.806 mg/g; β=0.0074 mg/g·h), respectively, were optimal for CoQ10 production. CoQ10 formation observed to be both growth and nongrowth associated. In optimized medium CoQ10 formation increased considerably from 1.91 to 14.12 mg/L.

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Authors and Affiliations

  1. Department of Biological System Engineering, Washington State University, PO BOX 646120, LJ Smith Hall, Pullman, WA, 99164-6120, USA

    Mahesh V. Bule

  2. Food Engineering and Technology Department, Institute of Chemical Technology, Matunga, Mumbai, 400019, India

    Rekha S. Singhal

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  1. Mahesh V. Bule
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  2. Rekha S. Singhal
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Correspondence to Mahesh V. Bule.

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Bule, M.V., Singhal, R.S. Fermentation kinetics of production of ubiquinone-10 by Paracoccus dinitrificans NRRL B-3785: Effect of type and concentration of carbon and nitrogen sources. Food Sci Biotechnol 20, 607–613 (2011). https://doi.org/10.1007/s10068-011-0086-6

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  • DOI: https://doi.org/10.1007/s10068-011-0086-6

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