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Evaluation of tool-like receptor-2 and 4 and interleukin-6 gene expressions in Turkish rheumatoid arthritis patients

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Abstract

Rheumatoid arthritis (RA) is a progressive inflammatory disease. Although the etiology and pathogenesis of RA are not known well, genetic and environmental factors are proposed to initiate an autoimmune process. We aimed to investigate mRNA expression levels of Toll-like receptor-2 (TLR-2), TLR-4, and interleukin-6 (IL-6) genes in RA disease. This study was conducted with 50 patients who were diagnosed with RA according to the American College of Rheumatology classification criteria for RA and 50 age-matched healthy control individuals who did not have any joint diseases and autoimmune diseases. We collected whole blood from all participants and analyzed expression of TLR-2, TLR-4, and IL-6 genes at mRNA level using real-time qPCR. TLR-2 expression was detected to increase 3.8-fold and IL-6 expression was detected to increase 6.8-fold in RA patients compared to healthy controls. No difference was found between patient and control groups with regard to TLR-4 expression. Overexpression of TLR-2 and IL-6 may be responsible for RA pathogenesis. Inhibition of both TLR and IL signaling pathways may prevent joint inflammation and destruction.

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Authors and Affiliations

  1. Department of Medical Genetics, Faculty of Medicine, Balıkesir University, Balıkesir, 10145, Turkey

    Betul Eser

  2. Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Balıkesir University, Balıkesir, Turkey

    Nilay Sahin

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  1. Betul Eser
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  2. Nilay Sahin
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Correspondence to Betul Eser.

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Written informed consent was obtained from all the patients. The study protocol was approved by the Ethical Committee for Clinical Research at Balikesir University.

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Eser, B., Sahin, N. Evaluation of tool-like receptor-2 and 4 and interleukin-6 gene expressions in Turkish rheumatoid arthritis patients. Clin Rheumatol 35, 2693–2697 (2016). https://doi.org/10.1007/s10067-016-3282-1

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  • DOI: https://doi.org/10.1007/s10067-016-3282-1

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