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Differential requirement of Oryza sativa RAR1 in immune receptor-mediated resistance of rice to Magnaporthe oryzae

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Molecules and Cells

Abstract

The required for Mla12 resistance (RAR1) protein is essential for the plant immune response. In rice, a model monocot species, the function of Oryza sativa RAR1 (OsRAR1) has been little explored. In our current study, we characterized the response of a rice osrar1 T-DNA insertion mutant to infection by Magnaporthe oryzae, the causal agent of rice blast disease. osrar1 mutants displayed reduced resistance compared with wild type rice when inoculated with the normally virulent M. oryzae isolate PO6-6, indicating that OsRAR1 is required for an immune response to this pathogen. We also investigated the function of OsRAR1 in the resistance mechanism mediated by the immune receptor genes Pib and Pi5 that encode nucleotide binding-leucine rich repeat (NB-LRR) proteins. We inoculated progeny from Pib/osrar1 and Pi5/osrar1 heterozygous plants with the avirulent M. oryzae isolates, race 007 and PO6-6, respectively. We found that only Pib-mediated resistance was compromised by the osrar1 mutation and that the introduction of the OsRAR1 cDNA into Pib/osrar1 rescued Pib-mediated resistance. These results indicate that OsRAR1 is required for Pib-mediated resistance but not Pi5-mediated resistance to M. oryzae.

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Authors and Affiliations

  1. Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, 446-701, Korea

    Min-Young Song, Chi-Yeol Kim, Muho Han, Hak-Seung Ryu, Sang-Kyu Lee & Jong-Seong Jeon

  2. National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China

    Li Sun & Zuhua He

  3. Department of Microbiology, Pusan National University, Busan, 609-735, Korea

    Young-Su Seo

  4. Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA

    Young-Su Seo, Patrick Canal & Pamela C. Ronald

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  1. Min-Young Song
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  2. Chi-Yeol Kim
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  3. Muho Han
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  4. Hak-Seung Ryu
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  5. Sang-Kyu Lee
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  6. Li Sun
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  7. Zuhua He
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  11. Jong-Seong Jeon
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Correspondence to Jong-Seong Jeon.

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These authors contributed equally to this work.

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Song, MY., Kim, CY., Han, M. et al. Differential requirement of Oryza sativa RAR1 in immune receptor-mediated resistance of rice to Magnaporthe oryzae . Mol Cells 35, 327–334 (2013). https://doi.org/10.1007/s10059-013-2317-6

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  • DOI: https://doi.org/10.1007/s10059-013-2317-6

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