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The rapid induction of OsPR1A protein is crucial in Xa21-mediated rice bacterial blight resistance

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Abstract

Rice XA21 is an immune receptor kinase that confers broad-spectrum resistance against Xanthomonas oryzae pv. oryzae (Xoo). Pathogenesis-related (PR) proteins are located downstream of the plant immune system and OsPR1A can be induced by Xoo. In this study, it was found that the OsPR1A protein was concentrated in the lesion region in rice leaves and barely detectable in the Xoo-free regions. The OsPR1A protein was detectable at 2 days post-inoculation (dpi) in 4021. In contrast, the expression of OsPR1A protein was detectable at 8 dpi in TP309. It indicated that the rapid induction of OsPR1A expression in 4021 is crucial for disease resistance. To investigate the function of OsPR1A protein in Xa21-mediated resistance, an RNA interference construct was transformed into 4021. The lesion lengths of OsPR1a-RNAi lines were shorter than that of TP309 and longer than that of 4021. OsPR1a-RNAi lines showed reduced OsPR1A abundance, in correlation with the compromised resistance to Xoo, supporting that OsPR1A play a positive role for Xa21-mediated resistance. These results enhance our understanding regarding the mechanism of Xa21-mediated resistance and the function of the OsPR1a gene.

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Acknowledgements

This study was supported by a grant from the Foundation for the Returned Overseas Scholars of Hebei Province (C20190335) and the National Natural Science Foundation of China for young researchers (#31400700).

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  1. The authors Gaowei Yan and Yuqing Liu contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Hebei Agricultural University, Baoding, 071001, China

    Gaowei Yan, Yuqing Liu, Jinping Lan, Tong Zhang, Tianxingzi Wang, Liyun Li, Guozhen Liu & Shijuan Dou

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  1. Gaowei Yan
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  4. Tong Zhang
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  7. Guozhen Liu
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Contributions

GL, SD, and LL conceived and designed research. GY, YL, JL, TZ and TW conducted experiments. GY, YL and GL analyzed data. GY, YL, GL and SD wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Guozhen Liu or Shijuan Dou.

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Yan, G., Liu, Y., Lan, J. et al. The rapid induction of OsPR1A protein is crucial in Xa21-mediated rice bacterial blight resistance. J Plant Pathol 104, 969–978 (2022). https://doi.org/10.1007/s42161-022-01105-2

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