Abstract
Photodynamic diagnosis is used during glioma surgery. Although some studies have shown that the spectrum of fluorescence was efficient for precise tumor diagnosis, previous methods to characterize the spectrum have been problematic, which can lead to misdiagnosis. In this paper, we introduce a comparison technique to characterize spectrum from pathology and results of preliminary measurement using human brain tissues. We developed a spectrum scanning system that enables spectra measurement of raw tissues. Because tissue preparations retain the shape of the device holder, spectra can be compared precisely with pathological examination. As a preliminary analysis, we measured 13 sample tissues from five patients with brain tumors. The technique enabled us to measure spectra and compare them with pathological results. Some tissues exhibited a good relationship between spectra and pathological results. Although there were some false positive and false negative cases, false positive tissue had different spectra in which intensity of short-wavelength side was also high. The proposed technique provides an accurate comparison of quantitative fluorescence spectra with pathological results. We found that spectrum analysis may reduce false positive errors. These results will increase the accuracy of tumor tissue identification.
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References
Stummer W, Stepp H, Moller G et al (1998) Technical principles for protoporphyrin-IX-fluorescence guided microsurgical resection of malignant glioma tissue. Acta Neurochir (Wien) 140(10):995–1000
Stummer W, Novotny A, Stepp H et al (2000) Fluorescence-guided resection of glioblastoma multiforme by using 5-aminolevulinic acid-induced porphyrins: a prospective study in 52 consecutive patients. J Neurosurg 93(6):1003–1013
Stummer W, Pichlmeier U, Meinel T et al (2006) Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial. Lancet Oncol 7(5):392–401
Chung YG, Schwartz JA, Gardner CM et al (1997) Diagnostic potential of laser-induced autofluorescence emission in brain tissue. J Korean Med Sci 12(2):135–142
Dailey HA, Smith A (1984) Differential interaction of porphyrins used in photoradiation therapy with ferrochelatase. Biochem J 223(2):441–445
Ishihara R, Katayama Y, Watanabe T et al (2007) Quantitative spectroscopic analysis of 5-aminolevulinic acid-induced protoporphyrin IX fluorescence intensity in diffusely infiltrating astrocytomas. Neurol Med Chir (Tokyo) 47(2):53–57
Toms SA, Lin WC, Weil RJ et al (2005) Intraoperative optical spectroscopy identifies infiltrating glioma margins with high sensitivity. Neurosurgery 57(4 Suppl):382–391
Lin WC, Toms SA, Johnson M et al (2001) In vivo brain tumor demarcation using optical spectroscopy. Photochem Photobiol 73(4):396–402
Savitzky A (1964) Smoothing and differentiation of data by simplified least squares procedures. Anal Chem 36(8):1627–1639
Wagnieres G (1998) In vivo fluorescence spectroscopy and imaging for oncological applications. Photochem Photobiol 68:603–632
Yin D (1996) Biochemical basis of lipofuscin, ceroid, and age pigment-like fluorophores. Free Radic Biol Med 21(6):871–888
Eldred GE, Miller GV, Stark WS et al (1982) Lipofuscin: resolution of discrepant fluorescence data. Science 216(4547):757–759
Zijlstra WG, Buursma A, van der Roest WPM (1991) Absorption spectra of human fetal and adult oxyhemoglobin, de-oxyhemoglobin, carboxyhemoglobin, and methemoglobin. Clin Chem 37(9):1633–1638
Faber DJ, Mik EG, Aalders MCG et al (2003) Light absorption of (oxy-)hemoglobin assessed by spectroscopic optical coherence tomography. Opt Lett 28(16):1436–1438
Faber DJ, Aalders MCG, Mik EG et al (2004) Oxygen saturation-dependent absorption and scattering of blood. Phys Rev Lett 93(2):028102
Louis DN, Ohgaki H, Wiestler OD et al (2007) The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol 114(2):97–109
Utsuki S, Oka H, Sato S et al (2007) Histological examination of false positive tissue resection using 5-aminolevulinic acid-induced fluorescence guidance. Neurol Med Chir (Tokyo) 47(5):210–213
Janzer RC, Raff MC (1987) Astrocytes induce bloodbrain barrier properties in endothelial cells. Nature 325(6101):253–257
Janzer RC (1993) The blood-brain barrier: cellular basis. J Inherit Metab Dis 16(4):639–647
Noguchi M, Aoki E, Yoshida D, Kobayashi E, Omori S, Muragaki Y, Iseki H, Nakamura K, Sakuma I (2006) A novel robotic laser ablation system for precision neurosurgery with intraoperative 5-ALA-induced PpIX fluorescence detection. MICCAI 4190:543–550
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This work was supported in part by grant for Translational Systems Biology and Medicine Initiative (TSBMI) and Grant-in-Aid for JSPS Fellows.
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Ando, T., Kobayashi, E., Liao, H. et al. Precise comparison of protoporphyrin IX fluorescence spectra with pathological results for brain tumor tissue identification. Brain Tumor Pathol 28, 43–51 (2011). https://doi.org/10.1007/s10014-010-0002-4
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DOI: https://doi.org/10.1007/s10014-010-0002-4