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Simultaneous detection of guanine and adenine in DNA and meat samples using graphitized mesoporous carbon modified electrode

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Abstract

A graphitized mesoporous carbon modified glassy carbon electrode (GCE/GMC) prepared by drop coating method without any pre-anodization of the underlying GCE or external binder/matrix, has been demonstrated for simultaneous electrochemical oxidation of guanine (G) and adenine (A) at oxidation potentials 0.60 and 0.85 V vs. Ag/AgCl, respectively, in the presence of thymine (T) by differential pulse voltammetric method in pH 7 phosphate buffer solution. Control voltammetric experiments with unmodified GCE, graphite nanopowder and multiwalled carbon nanotube modified electrodes yielded either feeble or with high-background current responses. Interestingly, the GCE/GMC showed highly efficient, stable and well-defined voltammetric signals. Thymine oxidation signal noticed discretely at 1.15 V vs. Ag/AgCl on the GCE/GMC was not influenced for the simultaneous determination of G and A. Constructed DPV calibration graphs were linear in the range of 25–200 and 25–150 μM, respectively, for the G and A. Corresponding detection limit (S/N = 3) values are 0.76 and 0.63 μM. Real sample analyses for the detection of G and A concentrations in calf-thymus DNA (detected [G]/[A] ratio = 0.82), beef brain and beef liver were successfully demonstrated with recovery values ~100 %.

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Acknowledgement

The authors are grateful for the financial support from the Department of Science and Technology (DST), Technology System Development Program, India.

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Authors and Affiliations

  1. Environmental and Analytical Chemistry Division, School of Advanced Sciences, Vellore Institute of Technology University, Vellore, 632 014, India

    Rajendiran Thangaraj & Annamalai Senthil Kumar

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  1. Rajendiran Thangaraj
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  2. Annamalai Senthil Kumar
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Correspondence to Annamalai Senthil Kumar.

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10008_2012_1895_MOESM1_ESM.doc

Supplementary data available for this article can be found in the online version. Individual and simultaneous DPV response at GCE/GNP for adenine and guanine (Fig. S1). Comparative simultaneous CV response of G, A and T at GCE/GMC and GCE/MWCNT in pH 7 PB solution (Fig. S2). (DOC 766 kb)

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Thangaraj, R., Senthil Kumar, A. Simultaneous detection of guanine and adenine in DNA and meat samples using graphitized mesoporous carbon modified electrode. J Solid State Electrochem 17, 583–590 (2013). https://doi.org/10.1007/s10008-012-1895-0

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  • DOI: https://doi.org/10.1007/s10008-012-1895-0

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