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Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode

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Abstract

Electrochemical DNA biosensor was successfully developed by depositing the ionic liquid (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), ZnO nanoparticles, and chitosan (CHIT) nanocomposite membrane on a modified gold electrode (AuE). The electrochemical properties of the [EMIM][Otf]/ZnO/CHIT/AuE for detection of DNA hybridization were studied. Under optimal conditions using cyclic voltammetry, the target DNA sequences could be detected in the concentration range of 1.0 × 10−18 to 1.82 × 10−4 mol L−1, and with the detection limit of 1.0 × 10−19 mol L−1. This DNA biosensor detection approaches provide a quick, sensitive, and convenient method to be used in the identification of Trichoderma harzianum.

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Acknowledgements

The authors would like to thank the Ministry of Science, Technology and Innovation, Malaysia and the Malaysian Genome Institute (MGI) for support under research project no. 001-002-0027.

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Authors and Affiliations

  1. Biotechnology Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, 88400, Malaysia

    Shafiquzzaman Siddiquee

  2. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, 43400 UPM, Malaysia

    Shafiquzzaman Siddiquee & Nor Azah Yusof

  3. Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, 43400 UPM, Malaysia

    Nor Azah Yusof, Abu Bakar Salleh & Fatimah Abu Bakar

  4. Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Serdang, Selangor, 43400 UPM, Malaysia

    Abu Bakar Salleh & Soon Guan Tan

  5. Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, 43400 UPM, Malaysia

    Fatimah Abu Bakar

Authors
  1. Shafiquzzaman Siddiquee
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  2. Nor Azah Yusof
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  3. Abu Bakar Salleh
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  4. Soon Guan Tan
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  5. Fatimah Abu Bakar
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Correspondence to Shafiquzzaman Siddiquee or Nor Azah Yusof.

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Siddiquee, S., Yusof, N.A., Salleh, A.B. et al. Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode. J Solid State Electrochem 16, 273–282 (2012). https://doi.org/10.1007/s10008-011-1322-y

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  • DOI: https://doi.org/10.1007/s10008-011-1322-y

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