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Characterization of single-stranded DNA on chitosan-modified electrode and its application to the sequence-specific DNA detection

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Fresenius' Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

Single-strand DNA could bind with chitosan on a platinum electrode via forming a tight DNA-chitosan complex. The salt concentration of the ssDNA solution had an obvious effect on the surface coverage, the immobilization was remarkably reduced at high salt concentration. The sample ssDNA immobilized on the chitosan-modified electrode can hybridize efficiently with the complementary sequences and be successfully used for the sequence-specific DNA detection. The same results could be obtained using a gold or graphite electrode modified with chitosan. The stability of this electrode has been also discussed.

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, Shanghai, 200062, P.R. China e-mail: yuzhi@online.sh.cn, , , , , , CN

    Chun Xu, Hong Cai, Qun Xu, Pingang He & Yuzhi Fang

Authors
  1. Chun Xu
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  2. Hong Cai
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  3. Qun Xu
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  4. Pingang He
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  5. Yuzhi Fang
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Additional information

Received: 11 October 2000 / Revised: 13 November 2000 / Accepted: 14 November 2000

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Xu, C., Cai, H., Xu, Q. et al. Characterization of single-stranded DNA on chitosan-modified electrode and its application to the sequence-specific DNA detection. Fresenius J Anal Chem 369, 428–432 (2001). https://doi.org/10.1007/s002160000673

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  • DOI: https://doi.org/10.1007/s002160000673

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