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Deinococcus radiodurans YgjD and YeaZ are involved in the repair of DNA cross-links

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Abstract

Orthologs of Escherichia coli ygjD and yeaZ genes are highly conserved in various organisms. The genome of the radioresistant bacterium Deinococcus radiodurans possesses single orthologs of ygjD (DR_0382) and yeaZ (DR_0756). Complete loss of either one or both genes did not result in any significant changes in cell growth efficiency, indicating that both genes are not essential for cell viability in D. radiodurans, unlike the case with other species such as E. coli, Bacillus subtilis and Saccharomyces cerevisiae. Survival rates following DNA damage induced by hydrogen peroxide (H2O2), N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), ultra violet (UV) radiation, γ-rays, cisplatin and mitomycin C (MMC) were compared among the wild-type strain and D. radiodurans ygjD/yeaZ null mutants. Cell viability of the null mutants did not decrease following exposure to H2O2 or MNNG. In addition, the reduction in cell viability following exposure to γ-rays, UV radiation or cisplatin was marginal in the null mutants compared to the wild-type strain. Interestingly, the null mutants exhibited high sensitivity to MMC, which mainly causes interstrand DNA cross-links. The sensitivity of the null mutants to MMC was restored to that of the wild type by transformation with plasmids expressing these genes. These results suggest that D. radiodurans ygjD and yeaZ genes are involved in DNA repair and play a role in the repair of DNA cross-links.

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Acknowledgments

I would like to thank Dr. Akira Nakamura, University of Tsukuba for the valuable comments and discussion about ygjD and yeaZ orthologs. I am also indebted to the members of Ion Beam Mutagenesis Research Group of JAEA, whose opinions and information have helped me very much throughout this study. This work was supported in partly by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (JSPS) (grant no. 22580098 to I.N.) and by the JSPS Research Fellowship for Young Scientists (to T.O.).

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Authors and Affiliations

  1. School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan

    Takefumi Onodera & Toshihiro Ohta

  2. Ion Beam Mutagenesis Research Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki, Takasaki, Gunma, 370-1292, Japan

    Takefumi Onodera, Katsuya Satoh & Issay Narumi

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  1. Takefumi Onodera
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  2. Katsuya Satoh
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Correspondence to Takefumi Onodera.

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Communicated by F. Robb.

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Onodera, T., Satoh, K., Ohta, T. et al. Deinococcus radiodurans YgjD and YeaZ are involved in the repair of DNA cross-links. Extremophiles 17, 171–179 (2013). https://doi.org/10.1007/s00792-012-0506-4

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