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Assessment of bone metabolism biomarkers in serum and saliva of cirrhotic patients

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Abstract

Objective

To assess the serum and salivary levels of biomarkers related to bone metabolism in cirrhotic patients as well as the evidence of osteoporotic changes on panoramic radiographs.

Materials and methods

Thirty-eight cirrhotic patients underwent anamnesis and physical examination. Specimens of blood and saliva were collected for evaluation by using Luminex™ xMAP technology to quantify RANKL, OPG, IL-1β, IL-6 and TNF-α. Panoramic radiographs were evaluated based on the mandibular cortical index (MCI) and the resulting data were compared to the expression of biomarkers in serum and saliva. Descriptive data analysis was performed and the Mann–Whitney’s test and Spearman’s correlation were used.

Results

Most of the sample consisted of males (68.4%) who had cirrhosis mostly resulting from alcoholism (28.9%). Median concentration values of RANKL (74.44 pg/mL), IL-1 β (45.91 pg/mL), IL-6 (67.69 pg/mL) and TNF-α (5.97 pg/mL) in saliva were higher than those observed in serum. In 72.7% of the panoramic radiographs, MCI was found to be suggestive of osteoporotic changes. No statistically significant correlation was observed between salivary and serum expressions of biomarkers or between biomarkers and MCI.

Conclusion

RANKL, OPG, IL-1β, IL-6 and TNF-α are expressed differently in serum and saliva and the concentration of these biomarkers is not related to MCI.

Clinical relevance

This study contributes to the study of the mechanisms of osteoporosis in cirrhotic individuals.

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Funding

This study was supported by grants from the Brazilian National Council for Scientific and Technological Development (CNPq grant no. 44004/2014–5), São Paulo Research Foundation (FAPESP grant no. 2015/07727) and the Coordination for the Improvement of Higher Education Personnel (CAPES Finance Code 001).

Author information

Authors and Affiliations

  1. Department of Stomatology, University of São Paulo School of Dentistry, Av. Prof. Lineu Prestes, 2227-Butantã, São Paulo, SP, 05508-900, Brazil

    Jefferson Rocha Tenório, Nathália Tuany Duarte, Natália Silva Andrade, Mariana Lobo Bergamini, Paulo Henrique Braz-Silva & Karem L. Ortega

  2. Department of Dentistry, Federal University of Sergipe, Lagarto, Sergipe, Brazil

    Natália Silva Andrade

  3. Laboratory of Virology, Institute of Tropical Medicine, University of São Paulo School of Medicine, São Paulo, Brazil

    Ana Carolina Mamana & Paulo Henrique Braz-Silva

Authors
  1. Jefferson Rocha Tenório
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  2. Nathália Tuany Duarte
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  3. Natália Silva Andrade
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  4. Mariana Lobo Bergamini
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  5. Ana Carolina Mamana
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  6. Paulo Henrique Braz-Silva
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  7. Karem L. Ortega
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Contributions

Conceptualisation—Jefferson Rocha Tenório, Karem López Ortega. Methodology—Jefferson Rocha Tenório, Nathália Tuany Duarte, Mariana Lobo Bergamini. Formal analysis and investigation—Jefferson Rocha Tenório, Natália Andrade, Ana Carolina Mamana Fernandes de Souza. Writing (original draft preparation)—Jefferson Rocha Tenório, Natália Andrade, Karem López Ortega. Writing (review and editing)—Jefferson Rocha Tenório, Paulo Henrique Braz-Silva, Karem López Ortega. Funding acquisition—Paulo Henrique Braz-Silva; Supervision: Karem López Ortega.

Corresponding author

Correspondence to Karem L. Ortega.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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The authors declare no competing interests.

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Tenório, J.R., Duarte, N.T., Andrade, N.S. et al. Assessment of bone metabolism biomarkers in serum and saliva of cirrhotic patients. Clin Oral Invest 26, 1861–1868 (2022). https://doi.org/10.1007/s00784-021-04161-7

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  • DOI: https://doi.org/10.1007/s00784-021-04161-7

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