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Osteoblast proliferation and differentiation on a barrier membrane in combination with BMP2 and TGFβ1

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Abstract

Objectives

Bioresorbable collagen membranes are routinely utilized in guided bone regeneration to selectively direct the growth and repopulation of bone cells in areas of insufficient volume. However, the exact nature by which alveolar osteoblasts react to barrier membranes as well as the effects following the addition of growth factors to the membranes are still poorly understood. The objective of the present study was therefore to investigate the effect of a bioresorbable collagen membrane soak-loaded in growth factors bone morphogenetic protein 2 (BMP2) or transforming growth factor β1 (TGFβ1) on osteoblast adhesion, proliferation, and differentiation.

Material and methods

Prior to experimental seeding, membranes were soaked in either BMP2 or TGFβ1 at a concentration of 10 ng/ml for 5 min.

Results

Human osteoblasts adhered to all soak-loaded membranes as assessed by scanning electron microscopy. Growth factors BMP2 and TGFβ1 increased osteoblast proliferation at 3 or 5 days post-seeding when compared to control collagen membranes. Analysis of real-time PCR revealed that administration of BMP2 increased osteoblast differentiation markers such as osterix, collagen I, and osteocalcin. BMP2 also increased mineralization of primary osteoblasts as demonstrated by alizarin red staining when compared to control and TGFβ1 soak-loaded membranes.

Conclusion

The combination of a collagen barrier membrane with growth factors TGFβ1 and BMP2 significantly influenced adhesion, proliferation, and differentiation of primary human osteoblasts.

Clinical relevance

The described in vitro effects following the combination of collagen barrier membranes with growth factors TGFβ1 and BMP2 provide further biologic support for the clinical application of this treatment strategy in guided bone regeneration procedures.

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Source of funding

No external funding, apart from the support of the authors' institution, was available for this study. We kindly thank Geistlich Pharma AG (Switzerland) for providing the barrier membranes used in this study.

Conflict of interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland

    Richard J. Miron & Anton Sculean

  2. Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland

    Richard J. Miron, Nikola Saulacic & Daniel Buser

  3. Department of Cranio-Maxillofacial Surgery, Bern University Hospital, Inselspital, Bern, Switzerland

    Nikola Saulacic & Tateyuki Iizuka

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  1. Richard J. Miron
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  2. Nikola Saulacic
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  4. Tateyuki Iizuka
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Correspondence to Anton Sculean.

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Miron, R.J., Saulacic, N., Buser, D. et al. Osteoblast proliferation and differentiation on a barrier membrane in combination with BMP2 and TGFβ1. Clin Oral Invest 17, 981–988 (2013). https://doi.org/10.1007/s00784-012-0764-7

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  • DOI: https://doi.org/10.1007/s00784-012-0764-7

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