Summary
The effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) and osteogenic revulsants alone or in combination at different time points and in different dosages on proliferation and osteogenesis of bone marrow stromal cells (BMSCs) in SD rats were investigated. Rat BMSCs were cultured in vitro and induced by rhBMP-2 in different dosages (10, 50, 100 and 200 μg/L) alone or in combination with osteogenic revulsants. MTT colorimetric assay was used to evaluate The proliferation, activity of alkaline phosphoric (ALP) and osteocalcin were measured at 3rd, 6th, 9th, 12th day respectively. The results showed that rhBMP-2 and osteogenic revulsants could promote the differentiation of BMSCs towards osteoblast phenotype. The proliferation of BMSCs could be enhanced by rhBMP-2 in a dose-dependent manner. The expression of osteoblast phenotype was significantly higher by using both of them than by using them alone, which was verified by the activity of ALP and osteocalcin. It was suggested that the combined use of rhBMP-2 and osteogenic revulsants could promote the proliferation and simultaneously induce and maintain the expression of osteoblast phenotype of BMSCs in rats.
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References
Prockop D J. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science, 1997,276:71–74
David C C, Reiner C, Carla M et al. Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proc Natl Acad Sci U S A, 2000,97(7):3213–3218
Noel D, Gazit D, Bouquet C et al. Short-term BMP-2 expression is sufficient for in vivo osteochondral differentiation of mesenchymal stem cells. Stem Cells, 2004,22(1):74–85
Lane J M, Yasko A W, Tomin E et al. Bone marrow and recombinant human bone morphogenetic protein-2 in osseous repair. Clin Orthop, 1999,361(4):216–227
Troen B R. Molecular mechanisms underlying osteoblast formation and activation. Exp Gerontol, 2003,38: 605–614
Chen D. Differential roles for bone morphogenetic protein (BMP) receptor type IB and IA in differentiation and specification of mesenchymal precursor cells to osteoblast and adipocyte lineages. J Cell Biol, 1998,13:295
Gronthos S, Zannettin A C, Hay S J et al. Molecular and cellular characterization of highly purified stromal stem cells derived from human bone marrow. J Cell Sci, 2003,116:1827–1835
Kim H, Lee J H, Suh H. Interaction of mesenchymal stem cells and in vitro osteogenesis. Yonsei Med J, 2003,44(2):187–197
Qian Q C, Liu Y P, Yang W D et al. The biological effects of recombinant human bone morphogenetic protein-2 and basic fibroblast growth factor on human bone marrow stromal cells. J Stomatology (Chinese), 2004,18(4):380–381
Lecanda F, Avioli L V, Cheng S L. Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. Cell Biochem, 1997,67:389–396
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Fang, H., Yang, X., Chen, A. et al. Effect of rhBMP-2 and osteogenic revulsants on proliferation and differentiation of bone marrow stromal cells in rats. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 27, 561–563 (2007). https://doi.org/10.1007/s11596-007-0522-2
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DOI: https://doi.org/10.1007/s11596-007-0522-2