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Biosynthesized silver nanoparticles prevent bacterial infection in chicken egg model and mitigate biofilm formation on medical catheters

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Abstract

Investigating the application of innovative antimicrobial surface coatings on medical devices is an important field of research. Many of these coatings have significant drawbacks, including biocompatibility, coating stability and the inability to effectively combat multiple drug-resistant bacteria. In this research, we developed an antibiofilm surface coating for medical catheters using biosynthesized silver nanoparticles (b-Cs-AgNPs) developed using leaves extract of Calliandra surinamensis. Various characterization techniques were employed to thoroughly characterize the synthesized b-Cs-AgNPs and c-AgNPs. b-Cs-AgNPs were compatible with human normal kidney cells and chicken embryos. It did not trigger any skin inflammatory response in in vivo rat model. b-Cs-AgNPs demonstrated potent zone of inhibition of 19.09 mm when subjected to the disc diffusion method in E. coli confirming strong antibacterial property. Different anti-bacterial assays including liquid growth curve, colony counting assay, biofilm formation assay supported the potent antimicrobial efficacy of b-Cs-AgNPs alone and when coated to medical grade catheters. Mechanistic studies reveal the presence of ferulic acid, that was important for the synthesis of b-AgNPs along with enhanced antibacterial effects of b-Cs-AgNPs compared to c-AgNPs, supported by molecular docking analysis. These results together demonstrated the effective role b-Cs-AgNPs in combating infections and mitigating biofilm formations, highlighting their need for further study in the field of biomedical applications.

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Schematic Illustration of Eco-Friendly Synthesis for Biofilm Prevention on Medical Catheters and Bacterial Infection Mitigation. Created with BioRender.com.

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Acknowledgements

LP and AU thank UGC, New Delhi for their junior research fellowship. Dr Sudip Mukherjee acknowledges director (Prof. Pramod Kumar Jain), Indian Institute of Technology (BHU) for providing research infrastructure and other facilities. SM further acknowledged research fund support from IIT (BHU) [OH-35-Other Capital/Miscellaneous Grant] and the Royal Society of Chemistry, UK (No. R22-2922732087) for providing funding support for this work. Authors acknowledges Vaishali Yadav and Dr. Bama Charan Mondal, Department of Zoology, Banaras Hindu University, Varanasi, UP, India for helping in the confocal microscopic analysis and generously providing GFP-E. coli, respectively.

Funding

IIT (BHU) [OH-35-Other Capital/Miscellaneous Grant] and the Royal Society of Chemistry, UK (No. R22-2922732087).

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  1. Lipi Pradhan and Prince Sah have contributed equally to this work.

Authors and Affiliations

  1. School of Biomedical Engineering, IIT (BHU), Varanasi, India

    Lipi Pradhan, Prince Sah, Malay Nayak, Anjali Upadhyay, Pragya Pragya, Gurmeet Singh, B. Mounika, Pradip Paik & Sudip Mukherjee

  2. Department of Physics, IIT (BHU), Uttar Pradesh, Varanasi, India

    Shikha Tripathi

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  1. Lipi Pradhan
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Contributions

LP and SM has conceptualized the idea of this manuscript. LP, PS, MN, GS, AI, PP have performed the experiments, developed the methodology, and validated the results. SM and LP has written the original draft of the manuscript and revised. SM has acquired the funding and supervised the overall work as the corresponding author.

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Correspondence to Sudip Mukherjee.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Dermal skin irritation study was performed after IAEC approval (IIT(BHU)/IAEC/2023/068; Approval Date: February 9, 2023).

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Pradhan, L., Sah, P., Nayak, M. et al. Biosynthesized silver nanoparticles prevent bacterial infection in chicken egg model and mitigate biofilm formation on medical catheters. J Biol Inorg Chem 29, 353–373 (2024). https://doi.org/10.1007/s00775-024-02050-4

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