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Sensitive and highly selective colorimetric biosensing of vitamin-C and vitamin-B1 by flavoring agent-based silver nanoparticles

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Abstract

A sensitive scheme was established for the detection of vitamin C (Ascorbic acid) and vitamin B1 (Thiamin HCl) using Maltol capped AgNPs (McAgNPs) as colorimetric sensor. The designed scheme showed an instant alteration in color from yellow to orange and green for vitamin-C and vitamin B1 sequentially. The probe was sensitive in a concentration range of (0–1 µM) with limit of detection 0.064 and 0.038 µM for vitamin C and vitamin B1 sequentially. The interaction mechanism between vitamin C and vitamin B1 and McAgNPs was evaluated by visible spectroscopy, FTIR, and AFM. Vitamin C attaches on the surface of nanoparticles by C=O group, while OH, C–S–C, and NH2 groups are involved in the binding of vitamin B1 with McAgNPs. The Vit-C/Vit-B1-McAgNPs complexes were stable over a wide range of pHs. The size of McAgNPs increased after the interaction of vitamin C/vitamin B1 from 30–40 nm to 500 and 400 nm sequentially. The scheme was successfully applied for the detection of vitamin C and vitamin B1 in urine, plasma, water, and commercial pharmaceutical tablets with good recoveries. The scheme was ascertained to be more sensitive than many other formerly described schemes.

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Funding

This research did not receive any specific grant from funding agencies the public, commercial, or not for profit sectors.

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Authors and Affiliations

  1. Department of Chemistry, Federal Urdu University of Arts, Science and Technology, Gulshan-E-Iqbal Campus, Karachi, 75300, Pakistan

    Syeda Sumra Naqvi, Humera Anwar & Asma Siddiqui

  2. H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan

    Muhammad Raza Shah

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  1. Syeda Sumra Naqvi
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  2. Humera Anwar
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  4. Muhammad Raza Shah
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Contributions

Conceptualization and supervision, SSN, and HA; methodology, SSN, and HA; validation, SSN, and HA; formal analysis, SSN, HA, AS, and MRS; resources, SSN, HA and MRS; data curation, SSN, HA, and MRS; writing-original draft preparation, SSN, HA; writing-review and editing, SSN, AS and HA.

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Correspondence to Syeda Sumra Naqvi.

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775_2022_1944_MOESM1_ESM.pdf

Supplementary file1Fig. S1a-b Stoichiometric ratio between McAgNPs and (a) Vit-C (b) Vit-B1. Fig. S2a-c AFM images of McAgNPs (a) Morphology and (b) 3-D image (c) 2-D image showing size of McAgNPs. Fig. S3a-b Visible spectral changes on Vit-C-McAgNPs and Vit-B1-McAgNPs complexes in the presence of (a) Lake water and Tap water (b) Urine and plasma. (PDF 686 KB)

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Naqvi, S.S., Anwar, H., Siddiqui, A. et al. Sensitive and highly selective colorimetric biosensing of vitamin-C and vitamin-B1 by flavoring agent-based silver nanoparticles. J Biol Inorg Chem 27, 471–483 (2022). https://doi.org/10.1007/s00775-022-01944-5

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