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The CoOOH-TMB oxidative system for use in colorimetric and test strip based determination of ascorbic acid

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Abstract

The authors report that cobalt oxyhydroxide (CoOOH) nanoflakes possess intrinsic oxidizing ability to directly oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to form a blue colored product (oxTMB) even in the absence of H2O2 and oxygen. In the presence of ascorbic acid (AA), less of the blue product is formed because AA reduces oxTMB. These findings constitute a new scheme for colorimetric detection of AA. Absorbance, best measured at 652 nm, linearly drops in the 10 nM to 1 μM AA concentration range, and the limit of detection is 5 nM (at an S/N ratio of 3). The reaction is complete within <5 s and highly selective. A strip test has been designed for fast and on-spot visual detection of AA. The method was applied to the direct estimation of AA in the microdialysate of brain, and also in soft drink samples. The strip test is considered to be a promising tool for the rapid screening of AA in brain and commercial samples.

Schematic of the CoOOH-TMB colorimetric system that exhibits a high selectivity for ascorbic acid (AA). A strip test has been designed for fast and on-spot visual detection of AA.

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Acknowledgements

This work was financially supported by National Natural Science Foundation (21575090), Beijing Municipal Natural Science Foundation (2162009), Youth Talent Project of the Beijing Municipal Commission of Education (CIT&TCD201504072) and Youth Innovative Research Team of Capital Normal University.

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Author notes

  1. Yongqi Ding and Junfeng Zhao are contributed equally to this work.

Authors and Affiliations

  1. Department of Chemistry, Capital Normal University, Beijing, 100048, China

    Yongqi Ding, Bo Li, Xu Zhao, Chao Wang, Minghui Guo & Yuqing Lin

  2. Zhengzhou Prevention and Treatment Center for Occupational Diseases, Zhengzhou, 450003, China

    Junfeng Zhao

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  1. Yongqi Ding
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  2. Junfeng Zhao
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  3. Bo Li
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  7. Yuqing Lin
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Correspondence to Yuqing Lin.

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Ding, Y., Zhao, J., Li, B. et al. The CoOOH-TMB oxidative system for use in colorimetric and test strip based determination of ascorbic acid. Microchim Acta 185, 131 (2018). https://doi.org/10.1007/s00604-018-2675-z

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