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Molecular and cellular mechanism of the effect of La(III) on horseradish peroxidase

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Abstract

Horseradish is an important economic crop. It contains horseradish peroxidase (HRP) and lots of nutrients, and has specific pungency. Lanthanum is one of the heavy metals in the environment. It can transfer through the food chain to humans. In this paper, the molecular and cellular mechanism of the toxic effects of La(III) on HRP in vivo was investigated with an optimized combination of biophysical, biochemical, and cytobiological methods. It was found that La(III) could interact with O and/or N atoms in the backbone/side chains of the HRP molecule in the cell membrane of horseradish treated with 80 μM La(III), leading to the formation of a new complex of La and HRP (La–HRP). The formation of the La–HRP complex causes the redistribution of the electron densities of atoms in the HRP molecule, especially the decrease in the electron density of the active center, Fe(III), in the heme group of the La–HRP molecule compared with the native HRP molecule in vivo. Therefore, the electron transfer and the activity of HRP in horseradish treated with 80 μM La(III) are obviously decreased compared with those of the native HRP in vivo. This is a possible molecular and cellular mechanism for the toxic effect of La(III) on HRP in vivo. It is suggested that the accumulation of La in the environment, especially the formation of the La–HRP complex in vivo, is harmful to organisms.

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Abbreviations

CV:

Cyclic voltammetry

DAB:

3,3-Diaminobenzidine

EMARG:

Electron microscope autoradiography

HRP:

Horseradish peroxidase

ICP-MS:

Inductively coupled plasma mass spectrometry

IEF:

Isoelectric focusing

MALDI-TOF/MS:

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

REE:

Rare earth element

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

TEM:

Transmission electron microscope

XPS:

X-ray photoelectron spectroscopy

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Acknowledgements

The authors are grateful for the financial support of the National Natural Science Foundation of China (20971069, 20471030) and the Foundation of State Developing and Reforming Committee (GFZ2071609). The authors are grateful to Erkang Wang (an academician of the Chinese Academy of Science), Shaojun Dong (an academician of the Third World Academy of Sciences) of Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Min Wang (an outstanding specialist in metal alloys) of Nanjing University of Aeronautics and Astronautics, and Chunhui Huang (an academician of the Chinese Academy of Science) of Peking University as their encouragement and valuable instructions have been very helpful in approaching this intriguing topic of interdisciplinary relevance. The authors thank the Research Center for Proteome Analysis, Key Lab of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing, 210046, People’s Republic of China

    Lihong Wang, Tianhong Lu & Xiaohua Huang

  2. The Key Laboratory of Industry Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Lihong Wang & Qing Zhou

  3. State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing, 100084, People’s Republic of China

    Xiaolan Ding

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  1. Lihong Wang
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  2. Qing Zhou
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  3. Tianhong Lu
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  4. Xiaolan Ding
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  5. Xiaohua Huang
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Correspondence to Xiaohua Huang.

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Wang, L., Zhou, Q., Lu, T. et al. Molecular and cellular mechanism of the effect of La(III) on horseradish peroxidase. J Biol Inorg Chem 15, 1063–1069 (2010). https://doi.org/10.1007/s00775-010-0665-7

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  • DOI: https://doi.org/10.1007/s00775-010-0665-7

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