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Effect of glucose on the morpho-physiology, photosynthetic efficiency, antioxidant system, and carbohydrate metabolism in Brassica juncea

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Abstract

The present experiment was conducted to investigate the promotive effects of exogenous glucose (Glc) on the morpho-physiology in Brassica juncea. L. cv. RGN-48. The plants were treated with the different concentrations (0, 2, 4, and 8%) of glucose as foliar spray at 25 days after sowing (DAS) for 5 days consecutively. The plants were collected to analyze various growth and photosynthetic parameters at 30, 45, and 60 DAS. After 5 days exposure to Glc, the level of carbohydrate, total reducing sugars, proline, plant water status, chlorophyll content, as well as that of activities of peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6), and superoxide dismutase (EC 1.15.1.1) were increased. Glc application also enhanced the gaseous exchange parameters, i.e., stomatal conductance (gs), internal CO2 concentration (Ci), transpiration rate (E), and net photosynthetic rate (PN) in intact leaf. Other enzymes, such as nitrate reductase (EC 1.7.99.4) and carbonic anhydrase (EC 4.2.1.1) were also increased. Additionally, microscopic studies further reveal a remarkable increase in the stomatal aperture on Glc exposure. Moreover, exogenous Glc decreases the levels of malondialdehyde (MDA), superoxide radical (O2·−) and hydrogen peroxide (H2O2). This indicates that exogenous Glc application has a positive effect on Brassica juncea plants.

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  1. Plant Physiology Section, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India

    Fareen Sami & Shamsul Hayat

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  1. Fareen Sami
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  2. Shamsul Hayat
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Correspondence to Shamsul Hayat.

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Sami, F., Hayat, S. Effect of glucose on the morpho-physiology, photosynthetic efficiency, antioxidant system, and carbohydrate metabolism in Brassica juncea. Protoplasma 256, 213–226 (2019). https://doi.org/10.1007/s00709-018-1291-4

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  • DOI: https://doi.org/10.1007/s00709-018-1291-4

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