Abstract
Dahlias that are naturally infected with potato spindle tuber viroid (PSTVd) do not exhibit symptoms. Therefore, if PSTVd isolates that are highly pathogenic in tomato plants infect dahlias, there is a significant risk of PSTVd infecting other plants via dahlias. In this study, we found that almost all highly pathogenic isolates were able to infect dahlia plants, but the symptoms varied depending on the cultivar. When mixed inocula composed of dahlia isolates and highly pathogenic isolates were tested, the dahlia isolates dominantly infected dahlia plants; however, the highly pathogenic isolates also coinfected plants. Our results also suggest that seed or pollen transmission from infected dahlia plants does not occur.
This is a preview of subscription content, log in via an institution to check access.
Data availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
References
Arezou Y, Jafarpour B, Rastegar MF, Javadmanesh A (2008) Molecular detection of potato spindle tuber viroid in Razavi and northern Khorasan provinces. Pak J Biol Sci 11:1642–1645
Behjatnia SAA, Dry IB, Krake LR, Conde BD, Connelly MI, Randles JW, Rezaian MA (1996) New Potato spindle tuber viroid and Tomato leaf curl geminivirus strains from a wild Solanum sp. Phytopathology 86:880–886
Boonham N, González L, Lilia Peralta E, Blockley A, Walsh K, Barker I, Mumford RA (2004) Development of a real-time RT-PCR assay for the detection of potato spindle tuber viroid (PSTVd). J Virol Methods 116:139–146
Bostan H, Gazel M, Elibuyuk IO, Caglayan K (2010) Occurrence of Pospiviroid in potato, tomato and some ornamental plants in Turkey. Afr J Biotech 9:2613–2617
Diener TO (ed) (1987). Plenum published corporation, New York
Fuji S, Mochizuki T, Okuda M, Tsuda S, Kagiwada S, Sekine KT, Ugaki M, Natsuaki KT, Isogai M, Maoka T, Takeshita M, Yoshikawa N, Mise K, Sasaya T, Kondo H, Kubota K, Yamaji Y, Iwanami T, Ohshima K, Kobayashi K, Hataya T, Sano T, Suzuki N (2022) Plant viruses and viroids in Japan. J Gen Plant Pathol 88:105–127
Güner Ü, Sipahioglu HM, Usta M (2012) Incidence and genetic stability of potato spindle tuber pospiviroid in potato in Turkey. Turk J Agric For 36:353–363
Matsushita Y, Kanda A, Usugi T, Tsuda S (2008) The first report of a tomato chlorotic dwarf viroid disease on tomato plants in Japan. J Gen Plant Pathol 74:182–184
Matsushita Y, Usugi T, Tsuda S (2010) Development of a multiplex RT-PCR detection and identification system for potato spindle tuber viroid and tomato chlorotic dwarf viroid. Eur J Plant Pathol 128:165–170
Nakahara K, Hataya T, Hayashi Y, Sugimoto T, Kimura I, Shikata E (1998) A mixture of synthetic oligonucleotide probes labeled with biotin for the sensitive detection of potato spindle tuber viroid. J Virol Methods 71:219–227
Nakashima A, Hosokawa M, Maeda S, Yazawa S (2007) Natural infection of Chrysanthemum stunt viroid in dahlia plants. J Gen Plant Pathol 73:225–227
Qiu C, Zhang Z, Li S, Bai Y, Liu S, Fan G, Gao Y, Zhang W, Zhang S, Lu W, Lu D (2016) Occurrence and molecular characterization of potato spindle tuber viroid (PSTVd) isolates from potato plants in North China. J Integr Agric 15(2):349–363
Owens RA, Verhoeven JTJ (2017) Viroids and Satellites). In: Hadidi A, Flores R, Randles J, Palukaitis P (Eds.), Chapter 14 – Potato spindle tuber viroid (pp. 149–158). Academic Press
Singh B, Kaur K (2014) Incidence of potato spindle tuber viroid (PSTVs) in potato growing areas of Punjab. Int J Plant Res 27:96
Tsushima D, Nishimura M, Toda T, Furuya H, Fuji S (2019) Molecular characterization and eradication of new potato spindle tuber viroid isolates from dahlia plants in Japan. Eur J Plant Pathol 154:1091–1102
Tsushima D, Fuji S (2022) Comparison of two highly sensitive methods to detect potato spindle tuber viroid in Dahlia using quantitative-reverse transcription-polymerase chain reaction assay. J Virol Methods 300:114401
Tsushima T, Murakami S, Ito H, He YH, Adkar-Prushothama CR, Sano T (2011) Molecular characterization of potato spindle tuber viroid in dahlia. J Gen Plant Pathol 77:253–256
Tsushima T, Sano T (2015) First report of Coleus blumei viroid 5 infection in vegetatively propagated clonal coleus cv. “Aurora black cherry” in Japan. New Dis Rep 32:7
Verhoeven JTJ, Westenberg M, Van Ede EPM, Visser K, Roenhorst JW (2016) Identification and eradication of potato spindle tuber viroid in dahlia in the Netherlands. Eur J Plant Pathol 146:443–447
Yanagisawa H, Shiki Y, Matsushita Y, Ooishi M, Takaue N, Tsuda S (2017) Development of a comprehensive detection and identification molecular based system for eight pospiviroids. Eur J Plant Pathol 149:11–23
Acknowledgements
This work was supported by the grant-in-aid “Regulatory research projects for food safety, animal health and plant protection” from the Ministry of Agriculture, Forestry, and Fisheries of Japan.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflicts of interest.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional information
Handling Editor: Jesús Navas-Castillo.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Tsushima, D., Fuji, Si. Infectivity of highly pathogenic isolates of potato spindle tuber viroid in dahlia. Arch Virol 168, 131 (2023). https://doi.org/10.1007/s00705-023-05761-2
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s00705-023-05761-2