Skip to main content
Log in

Identification and eradication of potato spindle tuber viroid in dahlia in the Netherlands

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

In 2013, potato spindle tuber viroid (PSTVd) was identified in 17 lots of seven dahlia cultivars at a single company in the Netherlands. Analysis of the genotypes showed highest identities with a PSTVd genome from dahlias in Japan. The dahlia isolate could be transferred experimentally to tomato and potato. In tomato this resulted in mild chlorosis and stunting; in potato no symptoms appeared, nor in aerial parts, nor in tubers. The infections in dahlias were eradicated by destroying all infected lots at the infested company. No infections were found in lots of the same cultivars grown by other companies. Moreover, PSTVd was not detected in any of the lots of three other companies that had bought dahlia planting material of the infested company. In 2014, PSTVd was not found in a survey including all cultivars in which PSTVd had been identified in 2013. Therefore, it was concluded that PSTVd had been eradicated in dahlias in the Netherlands.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

References

  • Boonham, N., Gonzáles Pérez, L., Mendez, M. S., Lilia Peralta, E., Blockly, A., Walsh, K., Barker, I., & Mumford, R. A. (2004). Development of a real-time RT-PCR assay for the detection of Potato spindle tuber viroid. Journal of Virological Methods, 116, 139–146.

    Article  CAS  PubMed  Google Scholar 

  • Botermans, M., Van de Vossenberg, B. T. L. H., Verhoeven, J. Th. J., Roenhorst, J. W., Hooftman, M., Dekter, R., & Meekes, E. T. M. (2013). Development and validation of a real-time RT-PCR assay for generic detection of pospiviroids. Journal of Virological Methods, 187, 43–50.

  • Di Serio, F. (2007). Identification and characterization of Potato spindle tuber viroid infecting Solanum jasminoides and S. rantonneti in Italy. Journal of Plant Pathology, 89, 297–300.

    Google Scholar 

  • Flores, R., Randles, J. W., Bar-Joseph, M., & Diener, T. O. (1998). A proposed scheme for viroid classification and nomenclature. Archives of Virology, 143, 623–629.

    Article  CAS  PubMed  Google Scholar 

  • Fujiwara, Y., Nomura, Y., Hiwatashi, S., Shiki, Y., & Itto, T. (2013). Pathogenicity of potato spindle tuber viroid isolated from Dahlia and its transmissibility in Dahlia. Research Bulletin of the Plant Protection Service Japan, 49, 41–46 (in Japanese).

    Google Scholar 

  • Luigi, M., Luison, D., Tomassoli, L., & Faggioli, F. (2011). First report of Potato spindle tuber and Citrus exocortis viroids in Cestrum spp. in Italy. New Disease Reports, 23, 4.

  • Mackie, A. E., McKirdy, S. J., Rodoni, B., & Kumar, S. (2002). Potato spindle tuber viroid eradicated in Western Australia. Australasian Plant Pathology, 31, 311–312.

  • Mertelik, J., Kloudova, K., Cervena, G., Necekalova, J., Mikulkova, H., Levkanicova, Z., Dedic, P., & Ptacek, J. (2009). First report of Potato spindle tuber viroid (PSTVd) in Brugmansia spp., Solanum jasminoides, Solanum muricatum and Petunia spp. in the Czech Republic. New Disease Reports, 19, 27.

    Google Scholar 

  • Owens, R.A., Flores, R., Di Serio, F., Li, S-F., Pallás, V., Randles, J.W., Sano, T., Vidalakis, G. (2012). Viroids. In King, A.M.Q., Adams, M.J., Carstens, E.B.,. Lefkowitz, E.J. (Eds). Virus taxonomy. Ninth report of the International Committee on Taxonomy of Viruses (pp. 1221–1234). London: Elsevier Academic Press.

    Google Scholar 

  • Rzhetsky, A., & Nei, M. (1992). A simple method for estimating and testing minimum evolution trees. Molecular Biology and Evolution, 9, 945–967.

    CAS  Google Scholar 

  • Schultz, E. S., & Folsom, D. (1923). Transmission, variation, and control of certain degeneration diseases of Irish potatoes. Journal of Agricultural Research, 25, 43–118.

    Google Scholar 

  • Shamloul, A. M., Hadidi, A., Zhu, S. F., Singh, R. P., & Sagredo, B. (1997). Sensitive detection of potato spindle tuber viroid using RT-PCR and identification of a viroid variant naturally infecting pepino plants. Canadian Journal of Plant Pathology, 19, 89–96.

    Article  Google Scholar 

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tsushima, T., Murakami, S., Ito, H., He, Y.-H., Raj, A. P. C., & Sano, T. (2011). Molecular characterization of potato spindle tuber viroid in dahlia. Journal of General Plant Pathology, 77, 253–256.

    Article  CAS  Google Scholar 

  • Van de Vossenberg, B. T. L. H., & Van der Straten, M. J. (2014). Development and validation of real-time PCR tests for the identification of four Spodoptera species: Spodoptera eridania, Spodoptera frugiperda, Spodoptera littoralis, and Spodoptera litura (Lepidoptera: Noctuidae). Journal of Economic Entomology, 107, 1643–1654.

    Article  PubMed  Google Scholar 

  • Verhoeven, J. Th. J., Jansen, C. C. C., & Roenhorst, J. W. (2008). First report of pospiviroids infecting ornamentals in the Netherlands: Citrus exocortis viroid in Verbena sp., Potato spindle tuber viroid in Brugmansia suaveolens and Solanum jasminoides, and Tomato apical stunt viroid in Cestrum sp. Plant Pathology, 57, 399.

    Google Scholar 

  • Verhoeven, J. Th. J., Jansen, C. C. C., Botermans, M., & Roenhorst, J. W. (2010). Epidemiological evidence that vegetatively-propagated, solanaceous plant species act as sources of Potato spindle tuber viroid inoculum for tomato. Plant Pathology, 59, 3–12.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank inspectors of the Flower Bulb Inspection Service and our organisation for sampling and staff of Naktuinbouw for performing the real-time RT-PCRs for screening on PSTVd.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to J. Th. J. Verhoeven.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Verhoeven, J.T.J., Westenberg, M., van Ede, E.P.M. et al. Identification and eradication of potato spindle tuber viroid in dahlia in the Netherlands. Eur J Plant Pathol 146, 443–447 (2016). https://doi.org/10.1007/s10658-016-0911-0

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-016-0911-0

Keywords

Navigation